Bicinchoninic acid assay

Acid forming elements are chlorine, phosphorus, and sulfur (different from alkaline-forming elements). These elements are the primary element in foods containing moderate to large amounts of protein (except for milk and a few other dairy products). I decided on using peanut oil because peanuts yield an acid, thus they contain acid-ash. Acid-ash residue is inorganic radicals that form acid ions in the body, causing lower pH making the urine more acidic. Acid urine favors the excretion of kidney…

a pH of seven, however most water, either from the tap or rain water, are not neutral (they do not have a pH of seven) instead it varies slightly and normally ranges from about 4.0 to 5.5 on the pH scale, which shows that they are slightly acidic. Acid rain is due to the chemical reaction that takes place…

Malachite Green Assay: The reaction that AGPase catalyzes produces PPi. The resulting PPi that is produced is hydrolyzed by an inorganic pyrophosphatase (Sigma Aldrich) to generate phosphate (Pi). Each reaction for the assay was conducted on a 96 well plate and the reaction mixture was 50µL and contained Pre-Mix which contained 50mM HEPPS, pH=8.0 (MP Biomedicals), 10mM MgCl2, 1.5mM FBP (Sigma Aldrich), and 0.2mg/mL Bovine Serum Albumin (Sigma Aldrich), and 1.5mU/µL yeast inorganic…

By using the bioinformatics information, we can determine that the non polar amino acids affect the bonding with hydrogen bonds. This means that the pH of the enzyme will be physiological. It can work better at a pH concentration of 7, and actually the cells can survive in that environment. Meaning, enzymes can work well with this concentration of pH, so it bonds do not break easily when it can be saturated of acid or base. 4. As our experiment says, the three different amylase will have…

a pH of around eight, or in other words the endpoint of a solution. It also allows us to see a physical change, thus resulting in a more accurate measure of how much was titrated. Citric acid is a weak acid, making it best titrated by using a strong base, such as sodium hydroxide. The combination of a weak acid and a strong base would result in an endpoint that corresponds to the range of phenolphthalein indicator approximately a pH of eight. Image A Phenolphthalein is composed of C14H20O4, as…

On the experiment 1 solvent abilities: All solutes were expected to dissolve in water because water is known as “universal solvent.” Observation showed that many salts and sugars were able to fully dissolve in the water solvent, but corn oil did not (Figure1).The reason salts would dissolve into water solvent because water molecule carried partial positive charge on hydrogen atom and partial negative charge on oxygen atom; they could react with cation and anion from salts that have ionic bonds…

This paper describes the process by which a three-component mixture was separated and identified. The mixture was separated with the aid of a separatory funnel and various solvents acids and bases. The compound was found to be comprised of meta-nitroaniline, naphthalene, and benzoic acid / 2-napthol. All three of them are organic compounds with melting points in the order they were listed 111-114, 79-82 and 121-122/121-123 °C. the percent yield of each component was also calculated at the end…

The experiment that I will be conducting focuses on how fast one tablet of Alka seltzer can dissolve in various temperatures of water. The core of this procedure is to determine the impact that temperature has on how fast something dissolves. My hypothesis for this experiment would be that the Alka Seltzer that dissolves the fastest would be the one that was placed in the water with the highest temperature. There will be controlled and manipulated variables explained throughout the experiment.…

3.2. Chemical quality parameters and pH value:- 3.2.1. Chemical quality properties, pH values and total plate count (TPC):- Total volatile basic -nitrogen (TVB-N), the peroxide value (PV), pH and total plate count (TPC) are good index to determine the freshness and quality of fishes and their products. Data presented in Table (4) show the values of TVB-N, PV,…

3.1: Ligand preparation: A series of benzimidazole derivatives were used to prepare the ligand dataset using benzimidazole as the query molecule. All the inhibitors selected comply with the Lipinski rule of five. A total of 50 molecules were taken up for the study. 3.2 Virtual screening: To further screen the compounds, additional similarity metrics were calculated by ROCS. ROCS is a shape based superposition method used to perceive similarity between molecules based on their three dimensional…