Assay

ELISA (Enzyme-Linked Immunosorbent Assay) ELISA is a technique to detect and quantify substances like proteins, antibodies, and hormones. The target antigen is immobilized to the bottom of the well and then an antigen-specific detection antibody is added. The detection antibody makes it possible to measure results. The three most common formats of ELISA would be direct assay, indirect assay, and sandwich assay. In direct assay, the antigen immobilizes to the bottom of the well where a labeled…

of them are pull-down assay, enzyme linked immunesorbent assay (ELISA), cross linking protein interaction analysis, etc. Pull-down assays are used for studying strong interactions using beaded support to purify interacting proteins. The main function of this technique is that it can confirm a predicted protein-protein interaction, but it can also identify novel interactions. After identification the purification consists of numerous wash and elution steps. In pull-down assays, because the…

included viral infection of cell cultures, hemagglutination assay, real-time quantitative PCR, plaque assay, transmission electron microscopy, TUNEL assay, flow cytometric analysis of apoptosis, western blot, subcellular fractionation extraction, inhibition of apoptosis using specific inhibitors, and cytokine and chemokine quantification. The replication efficiency of H1N1, H5N1, and H7N2 in P815 mast cells was analyzed in figure 1. A HA assay examined red blood cells’ contact with IAV’s…

Maximization Test and the Local Lymph Node Assay. In addition, the Buehler method can be used for topical devices only (i.e., those in contact with skin), per ISO 10993-10. Guinea Pig Maximization Test (GPMT) For this test, male and/or female healthy young adult animals are used. If female animals are used, the animals should be nulliparous and non-pregnant, as pregnancy reduces the ability of a female animal to detect a sensitization response. Assays with positive controls using the same…

Introduction: Planarians are free-living aquatic flatworms that posses remarkable regeneration abilities in addition to its distinct negative phototaxis behavior. A wealth of research studies has thoroughly examined its photophobic behavior under different wave frequencies and light conditions. Planarian are highly sensitive to white light and wavelengths of high frequencies, blue-green range ( Paskin TR, 2014). Two basic pigment-cup eyes situated in the head region govern its photophobic…

Troponin Results from High-Sensitivity Assays in Chronic Kidney Disease without Acute Coronary Syndrome, (diFilippi et al 2012) that accurately states the clinical problem of high troponin levels in patients with chronic kidney disease and answers the clinical question of “ In patients diagnosed with chronic kidney disease not currently experiencing active acute coronary syndrome, which troponin marker (cTnT), or (cTnI) result in a high sensitivity assay in the stable outpatient arena.” The…

parameter except run time. Run time may be adjusted based on the above analysis. To demonstrate the method of precision six samples were prepared as per the test method representing a single batch. Determine the assay of these samples and evaluate the precision of the method by the % RSD of the assay results. Table 3 Precision – Sequence of Injection S.No. Name of the Sample No. of Injections 1 Blank 1 2 Standard 1 6 3 Standard 2 2 4 Sample 1 2 5 Sample 2 2 6 Sample 3 2 7 Sample 4 2 8 Sample 5…

Two chromatographic methods were developed for the assay of the FDA approved lozenges containing dextromethorphan hydrobromide (DXT) and menthol (MNT). The first was a green HPTLC method which depends on using Merck HPTLC aluminum sheets of silica gel 60 F254 and methanol-ammonia (10:0.1, v/v) as mobile phase. This was followed by densitometric measurements of the spots which were retained at 0.28 ± 0.01 for DXT and 0.76 ± 0.02 for MNT scanned at 210 nm. The method was found linear over the…

DM and GalA content were determined according to the method reported by Aina et al., [32] and Food Chemical Codex 1996 [33]. 0.1g of pectin was weighed into a 250 mL conical flask, then wetted with 5mL ethanol. 100mLof deionized water was added, then six drops of phenolphthalein (0.1% in ethanol) indicator to each sample. Pectin dispersions were stirred until it was fully dissolved, thereafter, the solution was titrated slowly with 0.1N NaOH until the colour of the indicator just changed to…

In “The Supermarket: Prime Real Estate” assay, the author main point is to provide a clear illustration of how supermarket operate, why does each of them is laid out much like another. The author also mentions how large amount of research have done of how to design supermarket. She also add that overall, supermarket design follows fundamental rules, all of them based firmly on extensive research; Place the highest-selling food departments in the parts of the store that get the greater flow of…