Thus, calculations (in appendix) were carried out separately for all three assays. More care should be taken when carrying out such an experiment.
Misreading of the quantities of products was what led to the inaccuracies in the results.
The rate of the reaction (the rate at which NADH/H+ is oxidized and the use of the lactose dehydrogenase) was proportionate to the specific activity of the serum glutamate-pyruvate aminotransferase. As glutamate pyruvate produced pyruvate, it reacted with NADH/H+ to form lactate and NAD+ at the same rate. Assay 3 had the fastest rate of reaction, and so had the largest value for its specific activity, at 0.01019 micromoles per milligram per minute. This supports the idea that the previous two assays had an insufficient amount of NADH+ added, which would hinder the reaction producing lactate and NAD+. Assay 1 and assay 2 had much slower reaction rates, at
7.9149 × 10 (-4) and 2.8444 × 10(-3) micromoles per milligram per minute respectively.
All other substances were the same, and added in the same quantities, so should not have affected the