Assay

% inhibition = [(A0-A1)/A0] x 100 where A0 was the absorbance of the control (l- ascorbic acid), and A1 was the absorbance in the presence of ethanol extract of sample or standards. Dpph assay: DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) free radical method is an antioxidant assay based on electron-transfer that produces a violet solution in ethanol . This free radical, stable at room temperature, is reduced in the presence of an…

comparing the results and their cytotoxicity with MTT assay of the MCF7 breast cancer cell line. The results will indicate if the drugs have similar effects on both breast cancer cells and zebrafish embryos. It is given that embryogenesis and carcinogenesis have similar molecular mechanisms, therefore it is expected that these cell types act somewhat similar. Their developmental process is expected to support the same results as the MTT assay results for the cancer cells. 2. Methods and…

Most proteins are enzymes, which have an essential role in biological catalysis by increasing the rate of a reaction. The experiment conducted included an enzyme assay with the enzyme catalase and the substrate hydrogen peroxide. To complete the assay, the catalase enzyme was added to the hydrogen peroxide buffered solution. Every thirty seconds, portions from the tube was removed and placed into the labeled tubes. Based on the degree in color of each sample, a different absorption value would…

amplification of multiple targets in a single PCR reaction. Indeed, multiplex PCR is a modification of conventional or realtime PCR in which two or more different PCR products are amplified within a single reaction (Henegariu, 1997). In a multiplexing assay, more than one target sequence can be amplified using multiple primer pairs in a reaction mixture. As an extension to practical use of PCR, this technique has potential to produce considerable time saving and effort within the laboratory…

cells/mL. The cell culture was then incubated at 37°C with 5% CO2 and subcultured every 2-3 days, when the cell concentration reached approximately 8 x 105 cells/mL. THP-1 cell based assay THP-1 cell based assay was performed to determine the immune modulatory effects of H. pylori and its derived products. In this assay, THP-1 cells were stimulated by 0.5 µg/mL (0.1 µg/well) Escherichia coli LPS, exposed to different H. pylori products at various product concentrations, and incubated for 22…

make sure it stays below 20 C. This was done until the solution was homogeneous. The solution was then transferred to a centrifuge tube and centrifuged. The supernatant was aliquoted and stored in a -20 C for use in future experiments. Enzymatic Assay of LDH by Spectrophotometry. One tube of tissue extract was thawed out on ice and a…

DPPH radical scavenging activity assay, Trolox equivalent antioxidant capacity (TEAC) and oxygen radical absorbance capacity (ORAC) while direct method or in vivo method usually using ferric ion reducing antioxidant power (FRAP) assay. The most common assay has been used for antioxidant analysis is by using DPPH radical scavenging activity assay. This assay…

There will be three assays will be made and tested with both known and unknown plasmid. The first assay contains both PGEX-F1 primer and PGEX-R primer. Both primers enable to do replication with PCR in both Watson(top) strand and Crick(bottom). The presence of Saw1 insertion gene will not matter in this assay. Therefore, the known and unknown will not show a difference in bands generated from the PCR and Gel electrophoresis. The second assay has PGEX-F1 primer and Saw1 primer. The…

Three differ assays were used and tested in this experiment: Antibacterial, anti-biofouling, and cytotoxicity assay (anticancer). Each assay used different microorganisms that were grown and tested in the lab, and were then introduced to various levels of synthesized AgNPs-- growing D. radiodurans strain R1 ATCCBAA-816, without…

This study compared the changes in gene expression within wing tissue of Little Brown Myostis infected with White Nose Syndrome to bats without White Nose Syndrome. As expected, Pseudogymnoascus destructans caused significant changes in gene expression within the tissue. Inflammatory responses paired with anti-fungal host responses were triggered by the introduction of Pseudogymnoascus destructans. Necessary chemokines (Small secreted proteins that induce a certain response) were produced in…