The assay was performed as described in section 3.2.2.2. P4 concentrations were analysed in spent culture media for cells cultured for 48, 96 and 144 hr in the present of melatonin 0, 20, 200 and 2000 pg/ml under both 5% O2 and air condition. Samples were diluted 100 fold in PBS and analysed in duplicate with standard (0, 0.5, 1, 2, 5, 10 and 20ng/ml), blank and quality control. To avoid variation among plates, all samples were run together. Inter and intra assay % CV was (11.1) and (5.1) respectively. 4.2.3.2 Oestradiol…
chamber (Stemcell Technologies, Canada) that was gassed with anoxic gas mixture of 5% CO2 and 95% N2 for 5 min [Zeng et al. 2012]. Control cell cultures were maintained in a normal oxygenated DMEM containing 25 mM glucose. After various time intervals, cell cultures were placed back to the normoxic condition with normal culture medium. For concurrent treatment, human APC (Haematologic Technologies Inc, USA) was present in the culture medium during OGD and reoxygenation; for control, only vehicle…
However, according to this paper, some of the limitations of ELISA based testing are the hourly delay; the time in which antigen binds to the coated antibodies. The excessive use of reagents to coat and wash the 96-well plate, and the 96-well plates it is self where the assay is performed on. Therefore, this article introduces 3D printing platforms for ELISA 96-well plates. With 3D plates, sensitivity of the assay increased as well as antibody binding time to the 96-well plates bay 2 to 3 folds…
volcanic field. It is typical to find lime calcareous soils throughout the dry hot desert. As mentioned before the Chihuahuan Desert is mainly surrounded by the Sierra Madre Oriental (east) and the Sierra Madre Occidental (west). There are many more mountain ranges that have built up over time, including: the Sierra del Carmen, the Organ Mountains, the Franklin Mountains, the Sacramento Mountains, the Sandia-Manzano Mountains, the Magdalena-San Mateo Mountains, the Chisos Mountains, the…
The region is tectonically characterized by the ~6 kilometer thick oceanic Nazca plate subducting underneath the continental South American plate at a convergence azimuth of ~ 78 ͦ and at a rate of 6.6 centimeters per year making the area one of the most active convergent margins on earth, typically experiencing a Mw > 8 earthquake every 10 – 20 years (Moscoso, et.al., 2011). Notable quakes in recent history include the 1960 Mw = 9.5 Valdivia earthquake which was the largest ever recorded,…
4.2.1. Material: Lead zirconate titanate (PZT) PZT is a short for lead zirconate titanate inorganic compound that has a chemical formula of Pb [ZrxTi1-x] O3 (0 ≤ x ≤ 1). PZT is made and composed of the two chemical elements lead and zirconium combined with the chemical compound titanate while formed under extremely high temperatures. The dielectric constant of PZT can range from 300 to 3850, depending upon orientation and doping. PZT possesses piezoelectric effect meaning that it changes shape…
Background The eastern side of the North Island relies on rainfall from cold fronts, which causes irregular patterns including moisture deficiencies and extreme conditions of temperature (Garnier, 1958). Garnier (1958) has found that the mountain ranges have formed a climatic barrier separating the west and east and resulting in the different climates and geomorphology: the Tararua range; the Ruahine; the Kaimanawa; and the Ruakamara Mountains (Garnier, 1958). The eastern north island, New…
The gas generation is due to E. coli metabolic processes that generates acid and changing the turbidity. 2. The Membrane Filtration Method did work as well. MF method can replace the MNP test for microbiological analysis of water sample. After the incubation of the mEndo plate for 24 hours it is perceptible the grown of E. coli colonies across the plate. The results of this method are shown in the figure 4. As it was used mEndo, just the E. coli colonies form bright green colonies with a…
discussion with the class. Ask if any students have ideas why the earths outer layer is constantly changing. Lesson: (20 minutes) This lesson will teach the student about fault lines and boundaries where two separate tectonic plates meet, and why volcanoes are formed. Begin with a brief introduction of the theory of plate tectonics. Explain to the class that plate…
The evidence of continent shape, rock formations, fossils, and Paleoclimate evidence are all good enough proof to accept this theory as true. Also, with this new information that Alfred Wegener failed to provide with his theory of why the continents move will complete the theory, making there no reason to deny this theory. Tectonic Plates have a large amount of scientific evidence proving that they are real and that they do move which explains why Pangaea eventually split into separate…