Macromolecules Made By Cells Lab Report

Improved Essays
Glucose is a monosaccharide which is a simple sugar make up of a single chain or ring of carbon atoms with hydroxyl groups attached. Their carbonyl group is what distinguishes them. It has a sweet taste and is soluble in water.
A nitrogen atom is present in amino acids but not in sugar molecules
Elements found in lipids are hydrogen, oxygen, and carbon.

Purpose
The purpose in this lab is to determine the presence of certain macromolecules made by cells by detecting carbohydrates, lipids and proteins with four different indicators.

Materials

Test tubes
Test tube rack
Benedict's solution
Iodine
Biuret reagent
White paper
Hot water bath
Vegetable oil
Glucose
Sucrose
Starch
Protein

Distilled water

Post-lab questions
A positive test for starch would be with iodine, because the starch turned black and opaque after the test and is the only substance out of the six to drastically change colour. Also the starch did not change much after the other tests
…show more content…
Also glucose did not change colour as much in the other tests.
The test for lipids is the paper test because lipids will not dry up easily, in fact it has still stained the paper by the end of class. Also the paper becomes translucent when stained by a lipid.
The test for proteins is the with the biuret reagent because immediately after adding it, it will turn purple. Although with the benedict's the protein also turned purple, it was after heating it up. As well benedict's is the test for glucose so the only test left is the biuret reagent for proteins
The purpose of the distilled water was to act as the controlled variable. In each test the water did not react with the solutions so it was used to compare the result of the distilled water with the other solutions to detect the difference in colour and transparency of the

Related Documents

  • Superior Essays

    MR test tube inoculated and incubated for 24 hours, ten to fifteen drops of methyl red indicator then added to the cultured. If it turned red then that was the positive for the presence of mixed acids. No color change was negative, however, if the media turned orange, it was inconclusive. VP test tube was inoculate and incubated up to 3 days, vortexed, added Barrits Reagent A (alpha-napthol) and Barrits Reagent B (potassium hydroxide). Red or pink color was positive reaction, no color change was negative, however it could take up to an hour to see the color change.…

    • 1514 Words
    • 7 Pages
    Superior Essays
  • Great Essays

    Amylase Experiment

    • 1123 Words
    • 5 Pages

    The errors of the experiment are unknown and cannot be accurately stated. The possible improvements for the experiment could include that there are more intervals of temperature tested, pH levels could be tested, and the concentration of amylase put into the glucose…

    • 1123 Words
    • 5 Pages
    Great Essays
  • Decent Essays

    An extension question to further investigate this topic is that are the test that were given work all the time. A person or group would repeat the test multiple times with the same contents and record see if it tested negative or positive for lipids, proteins, carbohydrate-glucose, and carbohydrate-starch. You would do the test ten times and see if you get the same result ten times in a row. If it does not happen that means that the test does not work all the time or something was did wrong when doing the…

    • 437 Words
    • 2 Pages
    Decent Essays
  • Improved Essays

    After the water was boiling, Benedict's reagent was placed in to the six test tubes up to two centimeters. The first color of Benedict's reagent was blue. As glucose levels rise, Benedict’s reagent changes in color gradually from blue to yellow to orange to red. The first three test tubes of the blood vessel samples taken from postprandial were placed in the hot water bath. The time was recorded, using a stopwatch, to accurately document when each sample turned red.…

    • 1489 Words
    • 6 Pages
    Improved Essays
  • Improved Essays

    Glucose was used as the positive control and turned yellow. Sucrose is a disaccharide that presented negative this is because there is no free aldehyde or ketone group. Most predictions aligned with the results, however, nonfat milk and cream presented negative results because they did not change. All three unknowns were positive for simple sugars. The data that was recorded, resulted in some error due to not mixing the contents well before heating them.…

    • 790 Words
    • 4 Pages
    Improved Essays
  • Improved Essays

    5 drops of bromothymol blue indicator was added to each tube. Drops of sodium hydroxide solution was added dropwise to both test tubes and gently mixed until the solutions turned blue. The observations were recorded. The tubes were set to incubate at room temperature for 20 minutes and checked at 5 minute intervals to record the observations.…

    • 1180 Words
    • 5 Pages
    Improved Essays
  • Decent Essays

    Biuret Lab Report

    • 369 Words
    • 2 Pages

    Firstly, During the procedure the purpose of this lab was to determine and identify if there is protein in water, apple juice, vegetable oil, potato, and egg whites. This was done by using the biuret solution. So this means if the material turns purple then we know it contains the protein, but if it doesn't turn out to be purple then it doesn't contain protein. The data in this experiment shows that water, apple juice, vegetable oil, and potato all did not contain protein this is because none of them turned purple. Although, the only one that did turn purple out of the five materials because of protein was egg whites.…

    • 369 Words
    • 2 Pages
    Decent Essays
  • Great Essays

    Egg White Lab Results

    • 1325 Words
    • 6 Pages

    This will raise the pH of the solution to alkaline levels. Next, we will place one of each test tube in the three different temperature water baths for 30 minutes. After 30 minutes, we will remove the test tubes from the water baths and add six drops of Biuret reagent to each tube and mix thoroughly. The Biuret reagent will test for the amount of peptide bonds present in the egg whites.…

    • 1325 Words
    • 6 Pages
    Great Essays
  • Improved Essays

    Fats: store energy, aid in development of cells, serve as components of hormones and vitamins, and protect organs. There are saturated and unsaturated fats. Examples are oil, wax, and butter. Question 2: Janine is correct, Brains cells use glucose to fuel cellular activities, the…

    • 915 Words
    • 4 Pages
    Improved Essays
  • Improved Essays

    If the sample stayed the same pale blue color, then there was no presence of reducing sugars. The starch powder was the only sample that did not change color. The egg beaters, lemon juice,…

    • 761 Words
    • 4 Pages
    Improved Essays
  • Improved Essays

    Iodine Test Lab Report

    • 501 Words
    • 3 Pages

    The experiment was conducted in order to discover the type of carbohydrate that made up the known and unknown solutions presented in the lab. After heating the monosaccharide solution containing the Benedict’s test, the change of color from blue to orange proved that monosaccharides showed a positive reaction to the test. When an Iodine test was conducted on the monosaccharide solution, the carbohydrate resulted in no color change from its original yellow/brown color, showing a negative reaction. In both the Iodine and Benedict’s test, a disaccharide solution tested negatively due to the fact that there was no color change in either test. A polysaccharide solution displayed a negative reaction to the Benedict’s test, but the solution portrayed a positive result to the Iodine test due to a color change to black.…

    • 501 Words
    • 3 Pages
    Improved Essays
  • Improved Essays

    Glucose, fruit sugar, and sugar are monosaccharides. Sugar, milk sugar,…

    • 250 Words
    • 1 Pages
    Improved Essays
  • Improved Essays

    Carbohydrates Research

    • 1225 Words
    • 5 Pages

    The properties of each substance test will be explained in the discussion. Depending on the properties of the substance the precipitate will be determined. The Ninhydrin reaction is used by scientist to know approximately the amount of amino acids is present in a substance. Amino acids plus ninhydrin react to give an aldehyde, carbon dioxide, ammonia and reduced ninhydrin. The reduced ninhydrin and the ammonia then reacts again with the ninhydrin to give a purple compound which is the positive test.…

    • 1225 Words
    • 5 Pages
    Improved Essays
  • Improved Essays

    Macromolecules Conclusion

    • 768 Words
    • 4 Pages

    The first test performed, Benedict’s Test, was done in order to determine if Unknown #1103 was a reducing sugar. When Unknown #1103 was tested with Benedict’s reagent it turned blue-green, indicating that it was not a reducing sugar. The next test performed, the iodine test, would determine whether Unknown #1103 contained starch. Upon mixing the iodine solution with Unknown #1103, it turned orange, meaning there were no starches in the Unknown #1103. The Biuret test was performed next in order to determine whether Unknown #1103 contained any proteins.…

    • 768 Words
    • 4 Pages
    Improved Essays
  • Improved Essays

    Carbohydrates

    • 1011 Words
    • 5 Pages

    Iodine/Potassium Iodide test tests for the presence of starch. Starch turns an intense blue-black color upon addition of iodine. In the absence of starch, the brown colour of the aqueous solution remains. Sudan III is a red dye used for staining fat globules. It has the appearance of reddish brown crystals.…

    • 1011 Words
    • 5 Pages
    Improved Essays