A nitrogen atom is present in amino acids but not in sugar molecules
Elements found in lipids are hydrogen, oxygen, and carbon.
Purpose
The purpose in this lab is to determine the presence of certain macromolecules made by cells by detecting carbohydrates, lipids and proteins with four different indicators.
Materials
Test tubes
Test tube rack
Benedict's solution
Iodine
Biuret reagent
White paper
Hot water bath
Vegetable oil
Glucose
Sucrose
Starch
Protein
Distilled water
Post-lab questions
A positive test for starch would be with iodine, because the starch turned black and opaque after the test and is the only substance out of the six to drastically change colour. Also the starch did not change much after the other tests …show more content…
Also glucose did not change colour as much in the other tests.
The test for lipids is the paper test because lipids will not dry up easily, in fact it has still stained the paper by the end of class. Also the paper becomes translucent when stained by a lipid.
The test for proteins is the with the biuret reagent because immediately after adding it, it will turn purple. Although with the benedict's the protein also turned purple, it was after heating it up. As well benedict's is the test for glucose so the only test left is the biuret reagent for proteins
The purpose of the distilled water was to act as the controlled variable. In each test the water did not react with the solutions so it was used to compare the result of the distilled water with the other solutions to detect the difference in colour and transparency of the