Amylase

Chemical reactions are vital to living cells. Many chemical reactions take long periods of time to transpire. Enzymes serve as catalysts to speed up biochemical reactions in other substances. Each enzyme interacts with only one specific substrate (molecule) in an organism or a family of related molecules. Enzymes are not changed or used up by the reactions they catalyze, but their effectiveness can be influenced by environmental factors such as the pH level of an environment. All enzymes have an…

fits into sucrase (enzyme) where hydrolysis occurs consequently splitting sucrose into glucose and fructose. However, in our experiment, we used the enzyme amylase which hydrolyzes starch into simple sugars like glucose. In the experiment, we prepared seven test tubes two of which were control. One control contained zero milliliters of amylase, one milliliter of water and three…

The aim of this study was to investigate the optimal temperature for α-Amylase to catalyse the hydrolysis of starch. After the implementation of the experiment, it was established that the hypothesis, that the ability of α-Amylase to act upon large linear carbohydrate polymers in starch will prove most effective at 37°C (the optimum human body temperature), was supported by the observed data. The hypothesis was justified based on the observation that the overall average of average colour…

presence of glucose among the fluids in between the intestine and circulatory system then there is to be a breakdown of starch molecules within the digestive tract . Materials: One small beaker, Two large beakers, Dialysis tubing, Plastic pipettes, Amylase, Cooked starch, Lugol’s solution, Glucose test strips, Tubing clamps Procedure: Once in groups and materials are obtained one is to place four full…

breakdown proteins. Starch and albumin were used as the substrate to test the enzymes amylase, pepsin, trypsin, and unknown enzyme T. Lugol’s solution was used to test for the presence of undigested starch, while Biuret solution and Ninhydrin solution were used to test for the presence of undigested protein and amino acids respectively. There were many key findings in this experiment, one of which is that amylase was able to digest starch at all pH conditions, as it showed a gold colour, while…

I expected that pepsin will destroy the structure of gelatin protein, because the pepsin broke down the primary structure of the gelatin polypeptide into amino acid monomers. The hydrochloric acid (HCL) expected to affect the ability of amylase to digest starch, because as it is an acid it effects on the protein that makes it unraveled as it breaks down the secondary structure which it contains hydrogen bonds. As I have done to experiment one titled Digestion of Proteins by Pepsin. The result…

reaction, they only catalyze one or a few types of reaction. One of important enzymes in human body and many other animals is amylase that hydrolyzes starch, a major part of human diet for many people in the world. There is a variety of amylase produced from many sources in our body such as salivary glands in the mouth, pancreas cells, and small intestines (Scott, 2014). Amylase initiates the breakdown of starch which is a common plant polysaccharide composed of repeating glucose monomers into…

was in a temperature of 4°C took the longest time for the starch to disappear (10 mins 30 seconds). This means that at the temperature of 4°C amylase activity are the slowest, it takes longer for the starch to get digested. On the other hand when amylase was in the temperature of 80°C in tube one, starch disappeared much quicker (1min). This means that amylase acts quicker in a higher temperature. The body temperature for human is between 36°C to 37°C, which means it utilizes digestion of starch…

digestive enzymes in the pancreas, stomach, small intestine, and mouth. Digestive enzymes break down the food we eat into nutrients so that our bodies can absorb them. There are different digestive enzymes that digest certain macromolecules such as amylase which digest carbohydrates, lipase which digest fats, protease which digest proteins, and disaccharides which digest sugars. For the experiment pancreatin, which is a combination of digestive enzymes is used. It is produced in the pancreas,…

begins in the mouth by salivary enzyme, amylase, the fruits starches are digested into maltose and disaccharide. As the food travels through the esophagus to the stomach, no significant digestion of carbohydrates takes place as the HCl in the stomach stops amylase from continuing to break down the molecules. Carbohydrate are further digested in the SI (duodenum). The chyme from the stomach enters the SI mixes with pancreatic juices also containing amylase, which digests the starch and glycogen…