The control was the first row for both amylases. At 0 min. neither one had synthesized anything so this served as the starting point. The independent variable was the temperature; this is what was being changed. The dependent variable was the amylase activity; this is what (should have) changed because of the temperature. The iodine was a marker. It showed whether starch was still present in the wells. From the results, starch was present in all the wells; it was present at every temperature and every time interval. The hypothesis was not supported because temperature did not affect any of the amylase; there was no activity.
The results from the group experiment for Aspergillus oryzae amylase concluded with no changes taking place in any of …show more content…
Amylase is an enzyme that breaks down starch. Among other factors, high temperatures denature enzymes. Through experimentation this was not observed. The results of the experiment resulted in flawed data. By human error, a mistake was made that affected how well the enzymes synthesized starch. Another possible factor that could have brought about the flawed results is a mistake in measurement, specifically in temperature. The goal was to have the optimal temperature present so that amylase for both bacteria and fungus could synthesize. If the temperature had been correct, then the results would have looked more like the expected results in Figures 4 and 5. This error was not limited to just this group, rather the majority of the class got these same flawed …show more content…
The errors made are something from which to learn from. Although the source of the error is not entirely clear, there are a few things that could have been done to prevent such a large margin of error. One could have been the accurate measurement of temperature for the water baths. They have to be representative of what will affect the enzyme accordingly. Another improvement would be the prevention of cross-contamination. A drop in the wrong place can skew the results. Human error is inevitable, but it is something from which to learn from. Although there was a large margin of error the results of this experiment conclude that the hypothesis is rejected. This experiment can be taken in other directions. There are other factors that affect enzymes, such pH and salinity. In what way would pH affect enzymes? Not only can optimal temperatures be explored for Bacillus licheniformis and Aspergillus oryzae amylase, but for other enzymes from other organisms as well. How would the optimal temperature for an enzyme from a human, a mammal (warm-blooded), compare to the optimal temperatures for an enzyme from a lizard, a reptile
The results from the group experiment for Aspergillus oryzae amylase concluded with no changes taking place in any of …show more content…
Amylase is an enzyme that breaks down starch. Among other factors, high temperatures denature enzymes. Through experimentation this was not observed. The results of the experiment resulted in flawed data. By human error, a mistake was made that affected how well the enzymes synthesized starch. Another possible factor that could have brought about the flawed results is a mistake in measurement, specifically in temperature. The goal was to have the optimal temperature present so that amylase for both bacteria and fungus could synthesize. If the temperature had been correct, then the results would have looked more like the expected results in Figures 4 and 5. This error was not limited to just this group, rather the majority of the class got these same flawed …show more content…
The errors made are something from which to learn from. Although the source of the error is not entirely clear, there are a few things that could have been done to prevent such a large margin of error. One could have been the accurate measurement of temperature for the water baths. They have to be representative of what will affect the enzyme accordingly. Another improvement would be the prevention of cross-contamination. A drop in the wrong place can skew the results. Human error is inevitable, but it is something from which to learn from. Although there was a large margin of error the results of this experiment conclude that the hypothesis is rejected. This experiment can be taken in other directions. There are other factors that affect enzymes, such pH and salinity. In what way would pH affect enzymes? Not only can optimal temperatures be explored for Bacillus licheniformis and Aspergillus oryzae amylase, but for other enzymes from other organisms as well. How would the optimal temperature for an enzyme from a human, a mammal (warm-blooded), compare to the optimal temperatures for an enzyme from a lizard, a reptile