Amylase

#2-Properties of Water 1.) Hypothesis and Null Hypothesis: Hypothesis: Adding starch will keep from diffusing across the membrane. On the contrary, amylase will cause the starch molecules to break apart into smaller molecules of glucose allowing diffusion of glucose and Lugol’s reagent across the dialysis tubing. Null Hypothesis: Adding amylase to the beaker will have no effect on starch molecules and diffusion of the glucose and Lugol’s reagent across the dialysis tubing will not be…

of High Fructose Corn Syrup (High Fructose Corn Syrup). Three different enzymes are used in the processing of corn to the HFCS end product. The first step in HFCS production, which involves an enzyme, is liquefaction. The used enzyme is an alpha-amylase. The purpose of this enzyme is to convert the starch slurry into shorter-chain-length dextrins. The…

Enzymes are organic catalysts that speed up reactions that occur by lowering the activation energy of the reactants. By lowering the activation energy, the chemical bonds are weakened, thus the reactions are much faster than without the catalyst. Enzymes are composed of proteins which shape dictates the function of the enzyme. On the enzyme it contains a part called the active site where the substrate binds and the chemical reaction undergoes. A substrate is the chemical involved in the…

experiment, dialysis tubing was used to simulate the semipermeable plasma membrane of a caterpillar’s intestine. Cooked starch was prepared and mixed with the enzyme amylase to simulate the mixing of the caterpillar’s saliva with a starch/leaf and then placed in the dialysis tubing to simulate the beginning of the digestive process. The enzyme amylase breaks down starch into glucose and that glucose then is able to permeate the membrane of the caterpillar’s intestine. Lugol’s solution is able to…

eventually causing the cell to shrivel up and shrink. Both of these results make it extremely important for animal cells to reach equilibrium or isotonic levels. Place 4 full pipettes of starch into a small beaker. Use a pipette to add 4 pipettes of α-amylase to the small beaker then stir. Open dialysis tubing from one end then tie the tubing with a clamp. Open the moist end and add 4 pipettes of the solution from the beaker into the tubing. Wipe clean the outside of the bag to remove any excess…

between starch and amylase? Background Information: Enzymes and the function of enzymes are extremely vital to the human body in regards to how quickly the body can break down the macronutrients of starch, lipids, and proteins. I chose to investigate the breaking down of a complex carbohydrate (starch) because due to their size and structure, starch is broken down more slowly than other substances such as sugars, therefore I was intrigued to investigate the extent to which amylase could break…

The purpose of performing physiological tests is to figure out certain characteristics of microorganisms or to reach a conclusion on what an unknown microorganism is. It can be also used to determine what is needed for bacteria to be cultured in or on certain media. Culture media can either be liquid or solid, and they can be used to identify certain characteristics of different bacterial species. This is indicated by the growth or lack of growth in or on certain media. Solid media can also be…

is present in urine, saliva or semen it may detect the trace amounts. The Acid Phosphatase, PSA, and Christmas Tree tests are specific for analyzing semen and will not react to other blood or saliva, but may react with vaginal fluids. Finally, amylase enzymes are present only in saliva and the small intestine. The Amylose Azuretest, starch-iodine test, and Phadebus test will not react with other bodily fluids, which would be evident in the first 10 minutes of the test (Magle Life Sciences,…

components of monosaccharides. Digestion begins in the mouth, where chewing and biting breaks down carbohydrates physically and chemically. The enzyme salivary amylase combines with food. When the food enters the stomach hydrochloric acid present causes hydrolysis of sucrose to glucose and fructose. The food passes to the duodenum where pancreatic amylase is secreted and has optimum PH, so most digestion of carbohydrates takes place in the small intestine. There are enzymes present in the brush…

different from one company and the other company. There are many uniqueness of formula from each company but generally, the process is divided into three processes. The first step is dough mixing. The starch inside the flour can be broken by the alpha-amylase and will produce dissolved amylose which is the enzyme substrate to the next amylose degradation process. Starch hydrolysis process has a very important role to dough’s rheology characteristic because amount of water will be bonded by…