Blood Evidence Analysis

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In order to determine the sequence of events that occurred at a crime scene it is important to be able to determine and identify certain bodily fluids versus non-human substances. Based on the location of the biological evidence and its DNA can provide crucial information about the crime scene, its victim, and the suspect(s). These tests divided between presumptive and confirmatory testing.
Blood evidence is the most common type bodily fluid found at a crime scene. Presumptive testing at the crime scene can determine whether blood is present and may require the use of multiple methods to come to a conclusion. Luminol is the most common type of test, which will present with a blue luminescence because of oxidation of hemoglobin. Another test similar to Luminol is Fluorescein, but requires an alternate light source to determine the existence of blood evidence. Other tests include enzymatic assays such as the Kastle-Meyer test that will turn evidence pink, Leucomalachite will turn green in the presence of hemoglobin in blood. Finally, other techniques such as Heme Select, HemaTrace, or ABA card may be utilized based on personal or department preference (Virkler & Lednev, (2009)
Laboratory testing to
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Although, it if blood is present in urine, saliva or semen it may detect the trace amounts. The Acid Phosphatase, PSA, and Christmas Tree tests are specific for analyzing semen and will not react to other blood or saliva, but may react with vaginal fluids. Finally, amylase enzymes are present only in saliva and the small intestine. The Amylose Azuretest, starch-iodine test, and Phadebus test will not react with other bodily fluids, which would be evident in the first 10 minutes of the test (Magle Life Sciences,

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