Amylase

FDE 310 Food Materials Laboratory Report Determination of Reducing Sugar Content INTRODUCTION The objectives of this experiment is to determine reducing sugar content of food sample by performing Fehling method and to identify invert sugar content of sugars by using polarimetry and also to learn property of invert and reducing sugar. Carbohydrases represent a large class…

The rheology characteristics of enzyme clarified beetroot juice with added additives such as maltodextrin (MD), inulin (INU) and their combination (MD+INU) was studied at a wide range of concentrations (0 to 20% dry solids) and temperatures (5 to 80°C) by controlled stress rheometer. The rheological parameter shear stress was measured upto the shear rate of 600s-1. The temperature dependency of Newtonian viscosity of beetroot juice was described by Arrhenius equation. The effect of added solid…

α-Lactalbumin, a multifunction protein in milk, forms an enzyme complex with galactosyltransferase that catalyzes the last reaction in lactose synthesis.1 α-Lactalbumin has one Ca2+ binding site and several Zn2+ binding sites as well as many intermediary folding states, making it ideal for research involving the effects of cation binding on protein interactions as well as research on protein misfolding.1 α-Lactalbumin gives milk an antimicrobial property, making it important for infant health,…

After the experiments we did in class, I came to the conclusion that when starch is in the mouth, enzymes in our saliva break it down and turn it into glucose. My first piece of evidence comes from the investigation where we chewed a cracker with starch for two minutes. As we chewed, the cracker’s taste changed from bland to sweet. As we chewed, the enzymes in our saliva broke down the starch molecules, which caused them to rearrange and create a new product: glucose. My second piece of…

Amylase is an enzyme that is specifically in charge of breaking down starches into sugar by using water (hydrolysis). In general amylase is present in all living organisms, but can vary between different species. Porcine amylases and the amylases that is found in humans has an 81.7% similarity (Argwall, 1987). In order to determine the optimal environmental conditions of porcine liver amylase the experiments conducted in this lab will test ph. (acidity)…

Amylase Laboratory Report Introduction: Enzymes are a catalytic protein in a quaternary structure, that helps with the acceleration of molecules called substrates to achieve its desired chemical reaction. In this lab we used a specific enzyme called amylase. According to the lab manual, amylase is an enzyme found in one’s saliva or the pancreas to accelerate metabolism and cause a spontaneous reaction that helps with digestion of a substrate called starch. In order for any polymer such as…

1. What enzyme in the infant is lacking? Explain your answer The lacking enzyme in the infant is glucose-6 phosphatase. When blood glucose is low, glycogen breakdown in liver produce glucose-6-phosphate. G6P release glucose into blood using glucose-6-phosphatase enzyme. This patient is lacking this enzyme so the blood glucose level is low. 2. What is the metabolic reason for elevated levels of blood lactate in this patient? What are the biochemical sources of lactate? Because of the deficiency…

The experiment conducted was to observe the enzyme catalysis of Horseradish Peroxidase with different determining factors. In this experiment the horseradish peroxidase enzyme should have an optimal temperature of 35°C, should have an optimal pH of 6, and should catalyze rapidly with the substrate and enzyme concentration. We were able to observe that the enzyme reacts best with its optimal temperature of 35° C and optimal pH of 6. Moreover, when there’s a high the substrate concentration and a…

Vibrio Fischeri, Rachel Brown: BM304 Revision Guide 2016 Physiology: Vibrio Fischeri are Gram negative, motile rods. One of the key features that can be found in the species Vibrio Fischeri is its bioluminescence. This bioluminescence comes from one of the products of a reaction catalysed by the enzyme luciferase (see biochemistry and metabolism). Vibrio Fischeri are easy to grow and they display chemotaxic behaviour. It is best to grow Vibrio Fischeri at 28 degrees Celsius. (DeLoney-Marino CR,…

question Even though Amylase can get denatured at high temperatures, a slight increase in temperature, should increase the rate of amylase reaction. Research title The effect of temperature on amylase activity. Word count-1025 Background research Enzymes are catalysts which speed up reactions in living organisms. Enzymes function by decreasing the activation energy for a reaction. Amylase is an enzyme found in human saliva and catalyses the hydrolysis of starch .Amylase like all…