Enzyme inhibitor

molecules that rise over the activation energy level the faster the reaction occurs. Catalysts lower the activation energy level allowing the reaction to occur more rapidly (Barnhart and Hopper 2011). Most catalyzes are also known as enzymes (Barnhart and Hopper 2011). Enzymes are designed to only be able to…

Introduction: Enzymes play a major role in biological systems to catalyze and control different reactions. The study of enzymes’ behaviors toward substrates and products is significant to understand various enzyme catalyzed reactions in living organisms. One of the most important enzymatic group is cytochrome P450 (CYP450) which contribute to the metabolism of many endogenous as well as exogenous substances such as drugs. Although there are several subtypes of CYP450 family, CYP450 1A1 is going…

3.1: Ligand preparation: A series of benzimidazole derivatives were used to prepare the ligand dataset using benzimidazole as the query molecule. All the inhibitors selected comply with the Lipinski rule of five. A total of 50 molecules were taken up for the study. 3.2 Virtual screening: To further screen the compounds, additional similarity metrics were calculated by ROCS. ROCS is a shape based superposition method used to perceive similarity between molecules based on their three dimensional…

of Ethiopia) evaluated in this study are well endowed with all of the essential nutrients required for human nutrition. Should also be cautioned as they have indicated high antinutrient content especially trypsin inhibitors and alkoloids. All the tested samples contained antinutrients in varying concentrations. Consumption of this plant with low concentrations of antinutrients may appear safe at face value, but it should be noted that consumption of these plant materials…

Effects of Different pH Solutions on Lactase. Caitlin Hathaway, 2016, Functional Biology, Texas State University, San Marcos, TX, 78666. Enzymes work at different rates in different environments. We investigated the relationship between pH levels and the ability for the lactase enzyme to catalyze reactions. Lactase was added to different levels of pH with substrate and their subsequent rates of catalysis were measured. Out of the four samples, the experiment showed that the lactase was most…

oxidative phosphorylation. Generates the majority of adenosine triphosphate known as ATP, for energy. The pellet will be formed after the 2nd centrifugation; the absorbance measured of the mitochondria will be low. Succinate dehydrogenase (SDH) is an enzyme located in the mitochondria membrane. FAD is present, it then turns the succinate to cause cellular respiration. SDH will replace with DCIP, which is an artificial electron acceptor. DCIP is used to measure mitochondrial activity in the…

pathways, $X$ and $Y$. $X^*$ acts as an inhibitor of the response $R^*$, and $Y^*$ acts as an activator of $R$. Ignoring the diffusive terms, these equations can be represented by the following matrices, Applying the program to this module, we reach the conclusion…

dehydrogenase (SDH) in various solutions, specifically those containing and not containing a reagent. The assigned reagent was malonate, which was previously hypothesized to be an inhibitor of SDH. Bovine liver was manipulated primarily through centrifugation, and mitochondrial fractions were then created to serve as the enzyme-rich solutions the reagent would act upon. To determine the protein concentration of the mitochondrial fraction, the Bradford assay was implemented; the result will…

levels react towards the enzyme catalase, found in beef liver, and will be discovered by the number of oxygen bubbles through the degradation of hydrogen peroxide. Hypothesis The higher the pH level, the more production of oxygen bubbles in the solution. Variables Could affect activity of catalase - Temperature of reactant vessel - Concentration of Hydrogen Peroxide/Catalase - pH level of Hydrogen Peroxide - Competitive/Non inhibitors/obstacle Independent…

Introduction Enzymes are specialized proteins present in all living organisms, whether animals, plants or microorganisms (Reece et al., 2012) .In addition, life would cease to exist without these vital organic molecules, as they are needed for metabolic processes, and other reactions and processes in the body (Reece et al., 2012). Sometimes some RNA molecules can act as enzymes (Reece et al., 2012). Enzymes as well as their substrates work best at optimal levels, and are affected by temperature…