Peroxidase Enzymes

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Enzymes are biological catalysts that speed up the various chemical reactions that happen inside our cells. For the enzymes to function well, they need to be in an environment with a specific set of conditions, other than that it will lead to the denaturation of the enzyme or will render it completely inactive. There are a lot of factors that impact enzyme activities, but in this experiment we examined the effects of 4 different factors which are temperature, Ph level, addition of the inhibitor and enzyme concentration. We supervise the enzyme peroxidase by observing the oxidation of the dye Guaiacol which produces water and a tetraguaiacol which has a brown color. We placed the enzymes in different temperature and changed its concentrations. …show more content…
We use various Ph level and different millimeters of pipets to fill up the test tubes according to a table that specified the amount each solution to be put in each test tube. After we have followed the procedures accordingly. We then poured the mixtures into a cuvette, which we later placed into the spectrometer and collected the data. We repeated this process for the rest of the paired tubes and saved the collected data on the desktop which we use for the graph.
For the third experiment we tested the inhibitor on the peroxidase enzyme activity. We mixed 5 drops of 1% hydroxylamine the inhibitor and 2% of enzyme extract into a test tube and let the mixture sit for a few minutes. We followed the rest of the experiments based on the instructions given in the table of contents. After we obtain our results.
Our last but not the least experiment is on the effects of enzyme concentration of enzyme activity. We obtain a beaker of each of the following solutions: Turnip Extract, Ph 5 buffer, and a Guaiacol. We followed the instructions given in the lab manual for this experiment. After the activities we calibrated the spectrometer using the LoggerPro 3.82 software on the computer to capture the

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