We use various Ph level and different millimeters of pipets to fill up the test tubes according to a table that specified the amount each solution to be put in each test tube. After we have followed the procedures accordingly. We then poured the mixtures into a cuvette, which we later placed into the spectrometer and collected the data. We repeated this process for the rest of the paired tubes and saved the collected data on the desktop which we use for the graph.
For the third experiment we tested the inhibitor on the peroxidase enzyme activity. We mixed 5 drops of 1% hydroxylamine the inhibitor and 2% of enzyme extract into a test tube and let the mixture sit for a few minutes. We followed the rest of the experiments based on the instructions given in the table of contents. After we obtain our results.
Our last but not the least experiment is on the effects of enzyme concentration of enzyme activity. We obtain a beaker of each of the following solutions: Turnip Extract, Ph 5 buffer, and a Guaiacol. We followed the instructions given in the lab manual for this experiment. After the activities we calibrated the spectrometer using the LoggerPro 3.82 software on the computer to capture the