Enzyme inhibitor

Objective: In-silico assessment analysis of Protein database entries of the Pf. lactate dehydrogenase (PfLDH) enzyme, considered as an important target for designing of anti-malarial drugs. Methods: PDB structures of the enzyme Pf. lactate dehydrogenase were downloaded from Protein Data Bank. These were subjected to ANOLEA energy assessment analysis and Swiss model was used to analyse Ramachandran plot. Results: The energy assessment analysis and analysis of Ramachandran plot were carried out…

Soundararajan et al. (2006) study on the effect of Aerva lanata aqueous suspension reduced the urinary levels of stone promoters such as oxalate, calcium, uric acid, phosphorus and protein and increased the content of stone inhibitors citrate and magnesium. The oxalate synthesising enzymes that is, lactate dehydrogenase and glycolic acid oxidase also lessened in the liver and kidneys. The histopathological studies showed dissolution of calcium oxalate stones compared to disease control…

The inhibitor DDX3 used in our laboratory was Human shDDX3 of lentivirus, and the control group was shLuc of lentivirus, which was transfected into the cells by infection. First, BHK-21 cell was cultured to 6 cm dish and cultured to a cell density of about 12%, then remove the medium and rinse twice with 1 ml of PBS. After removing the PBS, then add lentivirus (shDDX3 and shLuc) (MOI = 50) and 3ml 2% RPMI (containing 1/1000 polybrene), then culture for 24 hours at 37 ℃ with 5% carbon dioxide…

unable to do the same. S200T does not change the functional group of the residue, so it may still be able to act as a nucleophile. However, it may be limited without the charge carrying role of histidine. For all these reasons, it is probable that the enzyme has decreased or no function as a protease. In an animal, this may result in a decreased ability to digest and absorb…

Experimental Method: Extracting LDH. The LDH used was extracted from bovine skeletal muscle tissue. In the cold room, a mass of 20 grams of muscle tissue was obtained. It was placed in a blender along with ice cold buffer, 50 mM sodium phosphate and 10 mM EDTA at pH 7.5. The mixture was then blended and homogenized in order to break open the cells; during this time the temperature was checked periodically to make sure it stays below 20 C. This was done until the solution was homogeneous. The…

Enzymes speed up the rates of most biochemical reactions by helping reactants interact with each other. Since enzymes are highly specific for a specific reaction, they only catalyze one or a few types of reaction. One of important enzymes in human body and many other animals is amylase that hydrolyzes starch, a major part of human diet for many people in the world. There…

Fermentation Rate in Different Sugars Abstract: This experiment is designed by students after studying the process of cellular respiration and fermentation. It demonstrates students’ understanding over the materials and utilization of the lab setting. In this experiment, the primary focus will on the speed of fermentation in different substances (sugars) in the same condition (temperature, volume, amount of yeast). Each solution will be mix with a fix amount of yeast and put in fermentation tube…

This bacteria had a positive catalase test meaning that it contains the enzyme catalase and was able to turn the hydrogen peroxide into water and oxygen and that this bacteria uses aerobic respiration. When plated on a mannitol/salt plate the bacteria was able to grow on the plate meaning that it has a resistance to high salt…

Results Subjects Eight subjects completed this study with a mean BMI of 22.18 ±2.9 kg/m2( range, 19.5-28.15),aged 22 ±1.2 year (range 21-24 year), fasting glucose concentration of high carbohydrate meal is 4.9 ±0.071 mmol ( range, 4.6-5.2 mmol) and fasting glucose concentration of high protein meal is 5.0± 0.098 mmol (range, 4.7-5.5 mmol).There is no significant differences between the two test meals in the fasting values. Metabolic response Results for plasma glucose is depicted in the…

Specific environmental conditions may play a role in how enzyme activity is affected. We conducted an experiment to observe whether the change in temperature altered the reaction rate of an enzyme. The activity rate of our enzyme, catechol oxidase, was collected by analyzing the changes in absorption rate of the enzyme in a buffered catechol solution after being placed in four different temperatures. In which one solution remained out in room temperature being to be our negative control. Each of…