The Effects of Competitive Inhibitors on Catechol Oxidase Enzyme Activity. Jaley Willingham, 2015, Functional Biology, Texas State University, San Marcos, 78666.
Abstract
Competitive inhibitors may have an effect on enzyme activity. We investigated the effects that parahydroxybenzoic acid has on the enzyme activity of catechol oxidase. For this experiment, different levels of parahydroxybenzoic acid concentration were added to catechol oxidase. Each level of concentration was then tested for the absorbance level in a spectrophotometer. After testing four different concentration levels of parahydroxybenzoic acid, it was concluded that the inhibitor blocked the majority of the catechol oxidase’s enzyme activity. Other than two slight mishaps, …show more content…
Competitive inhibitors specifically work by preventing the development of enzyme-substrate complexes because they have a similar form to the substrate particle (Day, 2015). In this case, the competitive inhibitor parahydroxybenzoic acid, also known as 4-hydroxybenzoic acid (National Center for Biotechnology Information), has a similar shape to catechol oxidase and the inhibitor competes with catechol oxidase for the active site. However, once the inhibitor has attached itself to the active site there is no reaction. The competitive inhibitor simply gets in the way of the enzyme activity. (Clark, 2009) The purpose of this experiment is to determine whether or not a competitive inhibitor can block the enzyme activity of a specific enzyme. Testing the effects of parahydroxybenzoic acid on the enzyme activity of catechol oxidase will provide a scientific answer to the hypothesis that parahydroxybenzoic acid is indeed a competitive inhibitor that will be able to block the enzyme activity of catechol …show more content…
The effect parahydroxybenzoic acid has on the absorbance/enzyme activity of catechol oxidase. The enzyme activity of catechol oxidase was measured using a spectrophotometer. The results from the four minute mark illustrate that each of the different concentrations of parahydroxybenzoic acid blocked the enzyme activity of the vast majority of the catechol oxidase.
References
Clark J. 2009 Oct 13. Enzyme Inhibitors. http://www.chemguide.co.uk [accessed Oct. 16,
Abstract
Competitive inhibitors may have an effect on enzyme activity. We investigated the effects that parahydroxybenzoic acid has on the enzyme activity of catechol oxidase. For this experiment, different levels of parahydroxybenzoic acid concentration were added to catechol oxidase. Each level of concentration was then tested for the absorbance level in a spectrophotometer. After testing four different concentration levels of parahydroxybenzoic acid, it was concluded that the inhibitor blocked the majority of the catechol oxidase’s enzyme activity. Other than two slight mishaps, …show more content…
Competitive inhibitors specifically work by preventing the development of enzyme-substrate complexes because they have a similar form to the substrate particle (Day, 2015). In this case, the competitive inhibitor parahydroxybenzoic acid, also known as 4-hydroxybenzoic acid (National Center for Biotechnology Information), has a similar shape to catechol oxidase and the inhibitor competes with catechol oxidase for the active site. However, once the inhibitor has attached itself to the active site there is no reaction. The competitive inhibitor simply gets in the way of the enzyme activity. (Clark, 2009) The purpose of this experiment is to determine whether or not a competitive inhibitor can block the enzyme activity of a specific enzyme. Testing the effects of parahydroxybenzoic acid on the enzyme activity of catechol oxidase will provide a scientific answer to the hypothesis that parahydroxybenzoic acid is indeed a competitive inhibitor that will be able to block the enzyme activity of catechol …show more content…
The effect parahydroxybenzoic acid has on the absorbance/enzyme activity of catechol oxidase. The enzyme activity of catechol oxidase was measured using a spectrophotometer. The results from the four minute mark illustrate that each of the different concentrations of parahydroxybenzoic acid blocked the enzyme activity of the vast majority of the catechol oxidase.
References
Clark J. 2009 Oct 13. Enzyme Inhibitors. http://www.chemguide.co.uk [accessed Oct. 16,