As a result, lactose is a competitive inhibitor. In the presence of the inhibitor, the slope of the Lineweaver-Burk plot becomes the equation below.
Using equation 7 along with the Km and the Vmax from series A, the Ki of Series B and C were calculated. The concentration of inhibitor for 50mM lactose was found to be 35714 μM using equation 1. Similar to the substrate concentration, the inhibitor concentration can be found using the same equation, but substituting in the inhibitor molar concentration and volume in place of substrate information. The inhibitor concentration was used along with the slope of series B to find Ki. A sample calculation is shown below. Then the same steps were used to calculate the Ki for series C. But, the inhibitor
Using equation 7 along with the Km and the Vmax from series A, the Ki of Series B and C were calculated. The concentration of inhibitor for 50mM lactose was found to be 35714 μM using equation 1. Similar to the substrate concentration, the inhibitor concentration can be found using the same equation, but substituting in the inhibitor molar concentration and volume in place of substrate information. The inhibitor concentration was used along with the slope of series B to find Ki. A sample calculation is shown below. Then the same steps were used to calculate the Ki for series C. But, the inhibitor