Lowest?
The highest was at pH 7, and the lowest was at pH 2. The catalase enzyme works the best at an optimal pH of 7 (neutral) and works the worst at an acidic pH of 2.
How does changing the pH affect the rate of enzyme activity? Does this follow a pattern you anticipated?
A change in pH not only affects the shape of an enzyme but it also changes the shape or charge properties of the substrate so that either the substrate cannot bind to the active site or it cannot undergo catalysis. If the enzymes pH is at an optimum level then the enzyme will work more efficiently so the rate of reaction will increase now as the pH changes away from the optimum the rate will decrease.
At what temperature is the rate of enzyme activity the highest? Lowest? Explain.
The highest rate of enzyme activity was the warm temperature. The warm was the highest because the enzyme was at a regular temperature where it was comfortable enough to have a good rate of reaction. The lowest one was hot temperature. This was because the enzyme was trying trying to move too fast and it was not able to react the right way because of the denatured