Enzyme

BIOLOGY LAB REPORT. ENZYME ACTIVITY Planning (a) – Pl (a): Research Question: How does the concentration of the salivary amylase affect its activity? Aim: To investigate the effects of concentration on the activity of salivary amylase Hypothesis: The lower the concentration of the salivary amylase, the less the enzyme activity and vice-versa is true provided that other factors such as temperature and the pH are kept constant. Variables: Independent variables: • the concentration of…

What predictions would you make about the enzyme activity if you were to continue to change each of the factors in Parts A – E? The predictions that would be concluded about the enzyme activity if it were to continue and change each of the factors in Parts A – E, would be that enzyme activity will decrease due to the optimal temperature, pH, concentrations, and metal salt concentration not being obtained. How does changing the concentration of enzyme affect the rate of decomposition of H2O2?…

Acid in the stomach functions to kill bacteria, and aid digestion by solubilizing food. The acid is also important to establish the optimal pH (between 1.8 and 3.5) for the function of the digestive enzyme pepsin. Stimulation of acid secretion involves the translocation of H+/K+-ATPases to the apical membrane of the parietal cell. When the cell is resting (not stimulated), H+/K+-ATPases are located in vesicles inside the cell. When the cell is stimulated…

Beakers 7 Method 7 Results 8 Discussion 9 Conclusion 10 Appendix 10 Calculations: 13 Bibliography 13 Introduction Enzymes are core components of reactions. Enzymes can be employed to hasten, or even delay reactions. Enzymes themselves are simply biological molecules (proteins) that affect a reaction as a catalyst. A catalyst is a chemical that induces a chemical reaction. Lipase is an enzyme that the human body uses to break down fats in foods so they can be absorbed in the intestines.…

DISCUSSION Nelson et al, 2008, state “all enzymes that exhibit a hyperbolic dependence of V0 on [S] are said to follow Michaelis-Menten kinetics”. The data gathered from this experiment and plotted in figure 6 shows that it is this pattern shown in the reaction of PNPP to PNP catalysed by acid phosphatase, where a hyperbolic curve is observed in the graph of rate of reaction against substrate concentration, approaching the Vmax asymptotically. Acid phosphatase behaved congruently to the…

Studying the reaction of the enzyme catalase in different temperatures The purpose of this experiment was to measure the speed and the effectiveness of the enzyme catalase. The enzyme catalase purpose is to break down harmful substances and turn them into oxygen and water. The substance in this environment was hydrogen peroxide, and the study was to see how well the chicken liver would work if it was in a boiled environment, room temperature environment, an acid immersed environment and a basic…

Background Information: Enzymes are catalysts for chemical reactions in living things, they help the body speed up the rate of virtually all of the chemical reactions that take place within cells.They are very important, enzymes are biological catalysts. Enzymes don’t need a lot of energy to start a chemical reaction, it all depends on the body's temperature. Enzymes need catalysts, catalyst is a substance that decreases the activation energy needed to start a chemical reaction. Enzymes are…

Enzymes, usually proteins, are biological molecules that increase chemical reactions. Enzymes are important for survival and they help out in several different functions throughout the body, such as assisting metabolism and digestion. Though not all, some enzymes take apart huge molecules into smaller parts which can be much simpler for the body to digest. For each experiment, I came up with a hypothesis before testing/knowing the results. When amylase influenced starch disappearance, I…

recombinant CYP3A4 and CYP3A5. A significant decrease in the luminescene activity in both recombinant CYP3A4 and CYP3A5 was observed in 10 µM and 100 µM of PTV (Fig 1). It seems that PTV is a candidate drug for metabolism against CYP3A4 and CYP3A5 enzymes. Metabolite profiling and Identification After incubating PTV with HLMs in the presence of a NADPH regenerating system, a metabolite (M2) of PTV was profiled, characterized, and identified by LC-MS/MS. A representative chromatograms are…

Amylase is an enzyme that breaks down starch. Among other factors, high temperatures denature enzymes. Through experimentation this was not observed. The results of the experiment resulted in flawed data. By human error, a mistake was made that affected how well the enzymes synthesized starch. Another possible factor that could have brought about the flawed results is a mistake in measurement…