Enzyme

Effect of temperature on alkaline phosphatase activity | |Enzyme Activity | |Temp |(nmoles pNP.min-1.mL-1) | |((C) | | |25 |15.43 | |30 |16.83 | |37 |19.61 | |45 |24.11 | |60 |14.73…

we must determine this by finding which enzymes contribute to this over-production, and the best way to find a component that is incorporated into a reaction is to remove that component and observe if the reaction still occurs. Therefore, the use of a library that contains a list of Francisella novicida mutants that lack one specific enzyme (or protein) would prove invaluable to use, as it can be used to determine with significant accuracy which enzymes are a requirement for this enzymatic…

in animals than plants, meaning that they would have a higher ammount of catalase. 2. Test tube 5 had a greater reaction rate than test tube 4. In test tube 4, there was a higher concentration of catalase (enzymes) than hydrogen peroxide (substrates). Even though the concentration of enzymes increased, the reaction rate was little to none. Because the…

where chewing and biting breaks down carbohydrates physically and chemically. The enzyme salivary amylase combines with food. When the food enters the stomach hydrochloric acid present causes hydrolysis of sucrose to glucose and fructose. The food passes to the duodenum where pancreatic amylase is secreted and has optimum PH, so most digestion of carbohydrates takes place in the small intestine. There are enzymes present in the brush border which converts disaccharides into monosaccharides see…

shown in Figure 1. The metabolically active coenzymes of riboflavin are riboflavin -5-phosphate or Flavin Mononucleotide (FMN) and flavin adenine dinucleotide (FAD) that participate in many vital oxidation reduction processes. Catalytic sites of some enzymes are covalently bound with riboflavin as prosthetic group. FAD is not strictly referred as a dinucleotide because the ribityl moiety is not attached to the isoalloxazine ring by glycosidic linkage.In FMN, the phosphoric acid is attached to…

glycolysis, using different tissues to compartmentalize opposing metabolic pathways. Uridine diphosphate (UDP) glucose is a nucleotide sugar used in many pathways. It is the precursor for glycogen and sucrose. It can also be used as enzymes to breakdown reactions. These enzymes are called UDP glucose pyrophosphorylase and UDP glucose dehydrogenase. The pathway that…

no molecules which fit the catalase enzyme and as such no reaction can take place. The reaction rate between the catalase and the hydrogen peroxide appeared to increase with the amount of available substrate (hydrogen peroxide). This is because catalase is capable of breaking down 40 million hydrogen peroxide molecules for every 1 catalase molecule and the more molecules of substrate present in the solution means that there are more molecules to fit the enzyme. The reaction results in more…

there for proteins are broken down into amino acids and then go through the anabolic reaction which builds them back up 8) Would you hypothesize that the meat tenderizer would still work if it were? Heated? Why? Meat tenderizer is an enzyme. Enzymes denature in extreme heat but work really good in room temperature 37c. So it depends what the temperature is. if it’s really hot around 60c Then it won 't work but if it 's room temp around 37 c then it will work. Liver Lab Analysis –…

The Effects of Competitive Inhibitors on Catechol Oxidase Enzyme Activity. Jaley Willingham, 2015, Functional Biology, Texas State University, San Marcos, 78666. Abstract Competitive inhibitors may have an effect on enzyme activity. We investigated the effects that parahydroxybenzoic acid has on the enzyme activity of catechol oxidase. For this experiment, different levels of parahydroxybenzoic acid concentration were added to catechol oxidase. Each level of concentration was then tested for…

common conditions, which affects millions of people. People with this condition do not produce the lactase enzyme which metabolizes the sugar on their own therefore they are unable to break down the Lactose which is sugar that is found in milk. Lactase is an enzyme found specifically in milk sugar “Lactose”. It breaks down the lactose into monosaccharaides, galactose and glucose. The Lactase enzyme can be used to aid people with this condition. Saladin, K. (n.d.). ANATOMY & PHYSIOLOGY: THE…