Enzyme

the enzyme concentration versus the reaction time of an enzymatic reaction, and the effects of the reactants and products concentration direction of the reaction. Specifically, in this lab the enzymes salivary amylase and phosphorylase were looked at the effect of the enzyme concentration versus the reaction time of an enzymatic reaction were observed by the iodine test along with the benedict’s test. Enzymes are known as biological molecules which catalyze different reactions and all enzymes…

Introduction: A human has thousands and thousands of different proteins, each with a specific structure and function. Along with their diverse functions, they vary in structure, each protein having its own unique three-dimensional shape. Proteins are polymers of amino acids joined together by peptide bonds. There are 20 different amino acids to make up all the different proteins on the earth. Each of the amino acids is composed of a central carbon bonded to a hydrogen, a carboxyl group, an amino…

Catalyst upsurge the reaction rate of biochemical reaction taking place in the interior of living cells without themselves undergoing any overall change. By having enzymes, they are needed to speed up reactions and they provide a different reaction pathway of lower activation energy require for the reaction to take place. Without these protein reactions the biological activities in our everyday live could cause severe…

What is a catalase? A catalase is an enzyme that destroys harmful substances inside your body, and is a form of protein. (Corsini, "What Is a Catalase?") What is an enzyme? Enzymes are substances that speed up chemical reactions. (Corsini, "What Is a Catalase?”) This experiment involved both of these two aspects. It was inferred before the experiment began that if the potato is placed in hydrogen peroxide at room temperature, then it will produce the most bubbling activity. The reason that the…

Km and Vmax determination of glucocerebrosidase towards p-NPG The Michaelis constant (Km) is a measure of the affinity of the enzyme towards the substrate, with smaller values representing greater affinity. Km and the maximum rate (Vmax) of leukocyte GCase were obtained through the Lineweaver-Burk plot (Figure 4) with artificial substrate p-NPG in concentrations from 0.71 mM to2.50 mM. Km and Vmax values for leukocyte GCase using p-NPG as substrate was found to be 12.6 mM and 333 U/mg…

Research Question: To what extent does temperature have an affect on the enzymatic rate of reaction between starch and amylase? Background Information: Enzymes and the function of enzymes are extremely vital to the human body in regards to how quickly the body can break down the macronutrients of starch, lipids, and proteins. I chose to investigate the breaking down of a complex carbohydrate (starch) because due to their size and structure, starch is broken down more slowly than other…

RNA to proteins. The type of protein created relies on what type of messenger RNA or mRNA that was produced during the transcription of DNA. In our experiment, we looked at the specific LacZ gene that codes for beta-galactosidase, which is a protein/enzyme that can break down lactose into its constituent monosaccharrides, glucose and galactose. The amounts of proteins produced by this gene were observed to see what strain would yield the most. Each of the strains tested had a mutation that would…

By “inactivating enzymes, alteration of drug targets and alteration of the ability of drugs to enter and or accumulate in its cells”, Enterobacter aerogenes has an interesting way of combating drugs (Enterobacter Aerogenes). This organism lives in “the endogenous human gastro-intestine”…

diversity, soil enzyme activities and crop yield could be affected by land management practices and all these soil properties were increased under intercropping systems than monoculture (Yang et al. 2013; Li et al. 2015b; Wang et al. 2014; Zou et al. 2011). Moreover, our previous study also discussed that soil biochemical and enzymes have direct effect by intercropping system…

friendly reducing agent in optimum time to easily exfoliate coating solution and substitute on the microelectrode. Series connected distributed sensing electrodes were fabricated and patterned with TRGO added platinum nanoparticles (PtNP), chitosan-enzyme composites, and nafion, which were integrated onto the modified surface for effective glucose sensing. The fabricated biosensor demonstrated good amperometric response to glucose with a sensitivity of 41.18 μA/mMcm2,…