Enzyme

Reviewing my data table and graph results it is clear to see that as the substrate concentration increases so does the rate of reaction. The catalysing of enzymes involves molecular movement of the substrate and enzymes in an aqueous solution. Therefore the substrate and enzymes are moving randomly within the solution (Brownian motion). By increasing the concentration of the substrate particles the rate of reaction is increased as the substrate is much more likely to find an active site. This…

remains. Enzyme and Substrate Solution Preparation A blank tube was prepared by adding 5 mL 0.08 M phosphate buffer (7.7 pH) and 0.5 mL of 2.5 nM ONPG to a cuvette to zero the spectrophotometer with the spectrophotometer set to 420 nm. The substrate solutions were prepared individually by adding 4.5 mL 0.08 M phosphate buffer (pH 7.7) to a Spec 20 cuvette with 0.5 mL of ONPG. The enzyme solution was prepared according to enzyme isolation process. Enzyme Assay 0.5 mL of the isolated enzyme was…

Which of the following is true? a. All enzymes are fibrous proteins. b. All enzymes are globular proteins. c. The majority of enzymes are fibrous proteins. d. The majority of enzymes are globular proteins. ANS: D PTS: 1 TOP: 23.1 - WHAT ARE ENZYMES? 2. In addition to proteins, which type of molecules sometimes function as enzymes? a. carbohydrates c. lipids b. DNA d. RNA ANS: D PTS: 1 TOP: 23.1 - WHAT ARE ENZYMES? 3. Which of the following is true of enzymes? a. They shift the position of a…

Making it obvious to observe the unexpected results as the temperatures 2, 20 and 27 remained blue then 37 degrees changed to green and 60 degrees changed to orange. This experiment was carried out to investigate how temperature has an affect on enzyme activity. This was done by preparing beakers with amylase, starch and benedicts solution at multiple temperatures and observing the colour changes present. The results collected from this experiment were…

interferes with the intracellular enzymes breaking down neurotransmitters. If FernaldiaTM can increase the number of intercellular enzymes breaking down neurotransmitters, it can also explain the decrease in the neurotransmitters in the vesicles and an increase in concentration of degraded neurotransmitters in the cytosol.…

temperatures at 4°C, 23°C, 32°C, and 48°C , pH 3, 5, 7, and 9, the boiled extract, and hydroxylamine had on the peroxidase enzyme extracted from Brassica rapa. Emma O’Donoghue Fundamentals of Biology I Lab Professor William Olsen October 8, 2015 Abstract The purpose of this lab was to determine the effects that a number of factors had on enzyme activity. The enzyme used during this lab was peroxidase, which was extracted from the organism Brassica rapa. The peroxidase was…

It is hypothesized that the optimum pH of trypsin is 8. Three different pH levels were tested, and each level consisted of three trials. The pH of 5 showed an average absorbance of 0.021±0.002, the pH of 8 showed an average absorbance of 0.167±0.030, and the pH of 11 showed an average absorbance of 0.088±0.001. Based on the standard deviation, there was a lack of variability between each trial for the pH levels of 5 and 11, yet the trials of the pH of 8 varied greatly compared to the other…

the presecnce of the citrase enzyme. H2S Production: The H2S test yielded a positive result, giving rise to a black precipitate created by iron and H2S combining. Proving the presence of the desulfurase enzyme. Indole: The indole test yielded a negative result, giving a yellow ring at the top of the test tube caused by lacking a reaction between Kovac’s reagent and indole, due to the fact that there is no indole present in the tube, as well as no tryptophanase enzyme. Methyl Red:…

indicates a constant enzyme reaction rate. The activity rate of catalase in figure 1 is calculated to be approximately 0.15mL/sec. The following figures show how this rate of reaction is affected by different external factors. The results in figures 2 and 5 show an increase in either enzyme concentration or substrate concentration will increase the enzyme activity. In figure 2, the 0.75% enzyme concentration had the highest enzyme activity with a rate of 0.11mL/sec; whereas the 0.25% enzyme…

reaction without being consumed in the process4. Enzymes are proteins that act as catalysts to a given biochemical reaction4. Enzymes manage to expedite metabolic reactions by lowering the activation energy required for the reaction to proceed4. In ideal conditions, the reactant macromolecule binds and acts on what is referred to as a substrate. However, enzymes require specific conditions specific conditions to perform most effectively4. Enzymes are susceptible to altering their optimal form…