Absorbance

Results After testing the absorbance of Control and Treatment with Penicillin and Streptomycin cultures over a 160-minute time period, three phenomena for the three different cultures were recorded and made into the graphs below. An upward trend of the absorbance in the Control culture as time progresses can be observed. Compared to the upward sloping absorbance curve of Control Treatment, both absorbance curves of Penicillin Treatment and Streptomycin Treatment have different directions of…

calculate peak wavelength of 409mn and zeroed with deionised water. A cuvette was filled with 0.139 mg/mL and the absorbance was measured, then again with the same concentration from the other test tube and an average was calculated. This was repeated for each concentration. Then a graph was plotted with concentration on the x-axis and the absorbance along the…

The process of photosynthesis is composed of two phases: light-dependent reactions and the Calvin cycle. The first stage (light-dependent reactions) occurs in the thylakoid membrane, which absorbs light energy to create NADPH and a proton gradient used to power the synthesis of ATP. The latter occurs in the stroma, and results in carbon dioxide fixation. In this experiment, only the light-dependent reactions were studied. 2,6-dichlorophenol-indophenol (DCPIP) was used as an artificial…

different products. In this experiment, we are using various techniques to calculate the protein concentrations of three different consumer products (Whole Milk, Protein Powder, and Muscle Milk) for comparison. Using spectrophotometry to find the absorbance of known protein concentrations, the collected data determines a…

states that absorbance, i.e. the amount of light absorbed by a solution, is equal to the molar absorptivity of a compound, times the path length of the cuvette, times the concentration of the solution. First, we measured the absorbance of a cuvette filled with water, then calibrated the spectrometer by subtracting this absorbance from our future measurements. Then, we measured the absorptivity of a 0.4 M solution of CuSO4 and found the wavelength at which this solution had an absorbance of 1.…

Both IR and UV spectroscopy are methods of measuring the wavelengths absorbed by a tested material. IR spectroscopy measures the absorbance of the tested material in the infrared spectrum, the section of the electromagnetic spectrum with a lower frequency than that observed in the visible light spectrum. Conversely, UV spectroscopy measures the absorbance of the tested material in the ultraviolet spectrum, the section of the electromagnetic spectrum with a higher frequency than that observed in…

effect of wavelength on T% and absorbance. The same equation used for Table 2 was used in Table 3 in order, to calculate the absorbance. The highest absorbance level was .300, which was at 400 nm, making 400 nm the analytical wavelength. Four different concentrations of Potassium Sulfate were than made and Table 4 demonstrated how the level of concentration of KH2PO4 affected T% and absorbance. The % transmittance ranged between 17.9%-66.3%, while, the absorbance ranged from .18 to .75. Graph…

observations, we calculated the change in absorbance for each tube, which can be seen in Graph 1. Calculating the change in absorbance better allows us to compare each tube to better understand which cofactor is needed in this enzymatic reaction. When referring to the data collected in our experiment, the main trend seen is that of either a positive or negative change in absorbance depending on the cofactor being tested in that tube. The change in absorbance was calculated by Tube 1 which…

525 nm. The calibration curve could then be created by measuring the absorbance of known concentrations, 5% to 25%, of cranberry juice at 525 nm, and generating a calibration curve of absorbance vs concentration. The absorbance of the cranberry apple juice was then measured at the wavelength of maximum absorbance. Absorbance is a measure of the amount of light that passes through a sample at a selected wavelength. The absorbance is measured by the spectrophotometer 20 which functions by…

enzymes chemistry is allowed in the cell. They do this by providing the energy needed for chemical bonds to break down and lets molecules interact with each other. For part 1 of the lab my hypothesis was if you increase the concentration, then the absorbance will also increase. For part 2 section A my hypothesis was if you increase the temperature, then the enzyme reaction time will also increase. For part 2 section B my hypothesis was if we change the level of the pH, then the ability of the…