Absorption spectroscopy

History of Atomic Absorption Spectroscopy (AAS) Atomic Absorption Spectroscopy (AAS) was first proposed by Alan Walsh, later known as Sir Alan in 1955. Sir Alan worked in Melbourne for the Commonwealth Scientific and Industrial Research Organization (C.S.I.R.O.). This concept came to Alan Walsh in a flash of inspiration as he was gardening at his Melbourne home. This led to the invention that has since been labelled as one of the most significant achievements in chemical analysis for last century. Atomic Absorption Spectroscopy(AAS) allows the measurement of extremely small amount of elements and is widely used in medicine, manufacturing, mining, environmental monitoring and laboratories all around the world. It is also used for the analysis of elements, particularly metallic elements, and is more commonly used than other methods. Therefore, it is also applicable to problems involving low concentrations of metal ions in aqueous solution. Not only has…

different concentrations over six orders of magnitude and also the BPES buffer, the different absorptions could not be measured and no calculations were possible. As a consequence, the binding constant of Ethidium Bromide to DNA using absorption spectroscopy was impossible to measure since no data was available. Instead, the whole experiment was redesigned by the TA in order to work which what was available at the lab and at least get a sense on how absorption spectroscopy works. Experiment was…

3. Objective In this project, a coordination compound, [Co(II)EDTA]2− will be prepared and its magnetic moment will be determined by NMR spectroscopy. A full picture of the clock behaviour will be presented through the combination of measurements provided by NMR spectroscopy alongside those obtained using more orthodox techniques such as UV spectrometry and pH measurements. The experiment in Dr. Evans paper will replicated using the same measurements and techniques. This project is to serve as a…

The optical bandgap (EQD) can be assessed using Tauc Relation equation (2). (αhυ)2 = f(R) hυ = B (hυ - EQD )n (2) where B is proportional constant (often negligible), hv is photon energy, EQD is bandgap energy, n based on the transition type (n = 1/2 for direct allowed transitions), and α is absorption coefficient. Kubelka-Munk Equation (3): approximation of α .5 …

3.4. Friction Characteristics of the NCD, MCD and boron doped NCD Coatings against Alumina Balls The friction coefficient curves of NCD, MCD and boron-doped diamond films sliding with Al2O3 balls are shown in figure 4, figure 5 and figure 6, respectively, for a sliding speed of 8 m/s and a load of 1N, 5N and 10N, respectively. Figure 4 (a), figure 5 (a) and figure 6 (a) show the friction coefficient of NCD, MCD and BDNCD at 1 N, respectively. Figure 4 (b), figure 5 (b) and figure 6 (b) show…

Abstract: The purpose of this lab was to show the different absorption and emission rates of three different substances. Radiation laws, albedo, and specific heat are all reasons why this is achievable. An agreed upon hypothesis is that the sand will absorb the most heat due to the number of molecules, the moss will absorb the second most because it doesn't have as many molecules as sand, but it has more than water, which isn’t as complex. To complete this experiment, you need three beakers of…

The purpose of the Determining Food Dye Content Lab was to identify the food dye coloring in green apple Gatorade, and determine the dye’s concentration to ultimately recreate a solution containing the same concentration of dyes as the original. To determine the concentration of the dyes present in the green apple Gatorade, a spectrometer was used to measure the wavelengths and absorbance values for the given dyes. A sample of the green apple Gatorade was tested in the spectrometer to measure…

Light spectroscopy has become an incredibly useful tool in analyzing the structure and composition of compounds. Specifically, the principles of absorption, the process by which an electron absorbs radiation to excite from a lower energy state to a higher energy state, and fluorescence, the emission of photons when a material is subjected to radiation, allow for the identification and analysis of unknown concentrations of compounds.1 With that being said, the purpose of this experiment is to…

criminal investigations, which can be aided by infrared spectroscopy. IR spectroscopy deals with the infrared region of the electromagnetic spectrum. This region is light with a longer wavelength and lower frequency than visible light. (Wikipedia) IR Spectroscopy is a method to identify substances based on their absorption of infrared wavelengths. It is a study of how the molecules of substances can absorb infrared radiation and convert it into heat. (Cole) A machine called an infrared…

of spectroscopy is to observe the relationship between substances in a solvent, and the associated absorbance and transmittance through a range of concentrations (Lothian & Twyman, 1969). However, one must understand the underlying principles of the electromagnetic spectrum in order to analyze the results of the techniques used in this experiment. When one notices the color of an object, they are observing the wavelengths of visible light – 400 nm to 750 nm approximately…