Absorbance

carrier was the phosphate buffer. The inhibitor that was used to test/slow the reaction was Malonate and the substrate used was succinate. In order to view the rate of metabolism that was occurring, we used a spectrophotometer to measure the color absorbance. When DPIP is reduced during the Krebs cycle, the color changes from blue to clear. The…

Throughout this lab, students worked with a common bacterium called Escherichia coli, which is a gram-negative bacterium that is found living in the digestive tracts of humans and animals. In the lab, various steps were carried out to evaluate and analyze the selective mediums that were created after doing our dilutions. Additionally, the number of colonies forming was calculated from specific time periods to establish a valid growth curve for E. coli. The growth curve’s shape and length is…

seen through the Beer-Lambert equation: A = Ɛ x b x c where (A) represents absorbance, (Ɛ) is the Molar absorptivity that is measured in M-1 cm-1, b symbolizes the distance the light travels, and c stands for the concentration. Based on these principles and processes, I hypothesize that the concentration of a substance and its absorbency are proportional in a sense that whenever the concentration increases, so will the absorbance of the…

spectrophotometrically to collect the data. The absorbance of these solutions was recording. However, the collected data should not be greater than the given absorbance of p-nitrophenol standard (1.310). If the absorbance of experimental solutions was higher than the standard one, that solution had to be diluted to get the lower number. The maximum time to dilute solution in this experiment was 2. According to the Beer-Lambert Law, the absorbance changes correspond to the concentration. It…

spectrophotometer. The higher the absorbance, the higher presence of Betacyanin pigments in the alcohol solution. My results for the 1-propanol solutions have an ascending absorbance from 0.001 to 0.364; however, this is after omitting the 40% alcohol concentration that has an unusual absorbance of 0.220.…

used to deduce the concentration of unknown proteins. A curve is produced by plotting concentration on X-axis and Absorbance on Y-axis. The unknown concentration can be found by reading their absorbance y axis, until intersecting the standard curve, and then reading down the graph until intersecting the x axis. According to Beer-Lambert’s law there is a linear relationship between absorbance and concentration, of an absorbing species. (David, 2001). Hence, the standard curve should produce a…

calibration curve had to be created by making up seven different solutions of eosin (11,25 μg ml-1) and water to compare it against their absorbance that was calculated in a spectrophotometer. Figure 1 shows this correlation. It is clearly evident that the higher the concentration of eosin in a test tube, the higher the absorbance of that test tube will be. It shows that absorbance is directly proportional to concentration. This…

methods that were followed during the process. A laboratory experiment was set up, using 5 samples of copper (II) sulfate solutions with concentration ranging from 0.080 to 0.4 mol/L. This was done in order to examine the relationship between the absorbance…

Discussion: Through experimentation, it has been determined spectroscopy provides valuable information on the absorbance behavior of triclosan in a solution. It acts as a viable way to measure the concentration of triclosan in a mixture. The data obtained using spectroscopy showed the soap sample contained 0.461% triclosan by mass. This coincides exactly with the information on the antibacterial concentration found on the bottle of soap. When using the spectroscopy method as a simple procedure…

Law1: iv) A=log⁡(l_t/l_o ) Where A is the absorbance of the sample, lt is the intensity of light passed through the solution and lo is the intensity of light before passing through the solution, which is the same for the same spectrophotometer.…