Absorbance

Several accusations were made that manufacturers were incorrectly labeling the iron content on their products. The purpose of this experiment was to analyze the amount of iron in approximately one serving of Total Whole Grain cereal and in one iron tablet and determine if these accusations were true. The values collected would then be compared to the actual values for these products. Total cereal contains 100% of the recommended daily allowance for iron, and the iron tablet contains 361% of the…

Light intensity was directly proportional to electron transport. As light intensity increased so did mean change in absorbance. The highest and lowest light intensities also had the highest and lowest mean change in absorbance. The more light that is readily available is a key factor in photosynthesis and electron transport. More light means more light energy which helps split water molecules at the beginning of electron…

(Biology Department, 2012). A greater absorbance reading is an indication that more products are released from the polysaccharide (Biology Department, 2012). It is hypothesized that enzymes and substrates function most effectively when subjected to moderate levels of temperature and pH. Likewise, when substrate or enzyme concentration is increased, a greater yield of products is the…

purpose of this experiment was to find and compare the amount of protein in Chobani Greek yogurt and Yoplait yogurt, to the standards given by the lab instructor. The two samples were diluted and added to the Bradford Reagent, which allowed for the absorbance of each sample to be found. The hypothesis was that, because Chobani Greek yogurt is thicker than Yoplait yogurt, it would have much more protein in it. The results showed that there wasn’t a big difference in the amount of protein between…

reaction activity over time and how substrate concentration affects their activity level. A supernatant was made through the dilution of blended potato extract. Then the supernatant was mixed with catechol and the reaction rate was measured in units of absorbance through a spectrophotometer every 30 seconds for ten minutes. The results supported the prediction that enzyme activity slows down over time. The next experiment done was to measure the effect of substrate concentrations. P-nitrophenol…

of the enzyme studied (Alkaline Phosphatase) were added upon insertion on the spectrophotometer apparatus. With intervals of 20 seconds their absorbance at a wavelength of 410 nm was recorded at time frame of 2 minutes for each solution. The initial velocity of the reaction (Vo) for each of the 5 samples was determined by plotting the change on the absorbance versus time. The slope was found from the…

The button mushroom had an absorbance rate of .0097 absorbance/min. Lastly, the oyster mushroom had the highest rate of reaction at .0328 absorbance/min. As the time increased the rate of absorbance increase, for example (Figure 1), there was a drastic increase with the rate of reaction of the shiitake mushroom from one minute to 6 minutes. Figure 1. The rate of cellobiase activity measured, in three different fungi types, in absorbance. The reaction rate was measure at 1,2,3,4…

Protein Purification III Parts 09/15/2015, 09/22/2015, 09/29/2015 Kaisha Butz Lab Partner: Ryan Van Abstract The lab was comprised of three parts, the purpose of which was to separate a protein mix and analyze the results. The first part was separating the proteins; the second part was reacting the proteins with an enzyme, and the third part was analyzing each fraction from Part I to see if separation was successful. It was hypothesized that out of the four proteins…

The only peak in absorbance is at the lowest end of the visible light spectrum, with a continuous decrease in absorbance as wavelength increases. This coincides with the observation of red as the color of the corresponding solution, since red is at the opposite end of the spectrum. Figure 4 is the spectrum of the cobalt complex with CO3 as its ligand. This complex was observed to be green. This is justified by the dip in absorbance for this complex around the 550 nm mark,…

blanked with the corresponding thiocyanate solutions. Time Duration For the time duration and wavelength determination, 5 mL of iron standard and 3 mL of saturated thiocyanate was mixed and diluted to 100 mL in a volumetric flask with 0.5 M HCl. The absorbance was read over a period of 2 hours at 30 min intervals. Excess Reagent Standard iron solution was used and 0.5 M potassium thiocyanate was prepared. Following the reagent table below, the corresponding amounts of each reagent was added…