Absorbance

Introduction Determining the concentrations of proteins is necessary for a variety of different reasons. For example, protein concentration must be known in order for proteins to be used in enzyme reactions, as the correct stoichiometry must be known. Another example is protein purification; during protein purification the quantity of protein present must be known to determine the yield1. The quantification of proteins is determined by methods known as protein assays. There are different…

also too see if the activity of the enzyme was influenced by the pH of the solution, which you will then see the results on the graphs and charts that I provided. In figure one we show results of the trial run from the enzyme activity where the absorbance was at 500nm.…

amount of product formed. To measure, a spectrophotometer monitors the colour intensity, which allows the determination of reaction rate. As the temperature increased, the average absorbance reading at 420 nm decreased, which is clearly depicted in Figure 1 below. The enzyme reached a maximum at its greatest absorbance point of 0.526 at 10°C, the lowest temperature tested. Enzymes are most active at their ideal temperature, allowing us to confirm that 10°C is the optimal temperature for…

Figure 2 The graph above (Figure 2) shows the relationship between increasing the amount of sodium polyacrylate in each pillow and the absorbance of Copper and Cobalt. The slope indicates that the more sodium polyacrylate added to each pillow the less Cobalt and Copper absorbed from the heavy metal ion solution. Figure 3 The graph above (Figure 3) represents the relationship between increasing…

most light. Catechol oxidase was tested with four different substrates to determine which produced the most benzoquinone. After preparing the spectrophotometer, I added solutions and then I let the substrates react for five minutes. I measured the absorbance of each set by zeroing the blank and testing its corresponding test tube and recording the data in a table. Pyrogallol produced the most benzoquinone and therefore benzoquinone absorbed the most light. My…

ions in an equilibrium reaction. The absorbance of each solution was found using a spectrophotometer, the first five of which was used to create a calibration curve, whose equation relates the concentration and absorbance of FeSCN2+ ions. Theories…

Iodide sample were determined by applying Beer’s Law in UV-Vis spectroscopy. Absorbance value of Cu-cuprizone solution was measured. Using the absorbance value of 0.3576 , the concentration of the Cu-cuprizone solution were obtained at 0.001389g/L with reference to the graph of the standard solution A to D. The graph of absorbance against concentration of the standard solution A to D shows a linear relationship hence absorbance is proportional to the concentration. This concentration obtained…

blood sample by measuring the absorbance of the sample as methemoglobin and cynamethemoglobin at 450-650nm. Furthermore, the hemoglobin and iron concentration were also measured by means of volumetric analysis and the Wong method. The concentration of Hemoglobin and iron obtained in the experiment was in good agreement with their corresponding literature value. The computation for the error analysis suggests that the error in the concentration with respect to the absorbance contributed highly to…

This was obtained by comparing the amount of phosphate to the absorbance that was calculated for each beaker. By analyzing the results of the Unknown beaker and comparing it to the national standard maximum in the United States, it is safe to say that the faucet used is safe for humans to use. The 0.3010 mg/L absorbance found for the Unknown was way less than the 3 mg/L, which is the United States standard maximum. It is important to note…

understanding of photosynthetic reactions by and experimenting with the factors that affect photosynthesis, which are light intensity, pigments, and light wavelength. By experimenting with these factors, our group was able to create an action and absorbance spectrum, which allowed us to determine which light wavelengths do the pigments in plants respectively absorb for photosynthesis. This allowed us to fully understand what conditions photosynthesis must…