Absorbance

The purpose of this experiment was to identify the different absorbance levels and response between normal cells and cancerous cells. Also, the difference between resting or stimulated cells and whether they have a difference in absorbance from each other. Inactivated forms of ERK cells don’t react and bind to the antibody on the ELISA plate, therefore this results in a no colour change on the plate. Whereas activated with added phosphate ERK cells react with the antibody and so antibodies do…

what the absorbance spectrum of Spinacia oleracea extract was and what the absorbance spectrum of the pigments were within the extract. From the use of the spectrophotometer I was able record the absorption spectrum of chlorophyll a, chlorophyll b, carotene, and xanthophyll to determine the range of wavelengths of light absorbed by the molecules. It was predicted within the Spinacia oleracea extract, chlorophyll a and…

It is hypothesized that the optimum pH of trypsin is 8. Three different pH levels were tested, and each level consisted of three trials. The pH of 5 showed an average absorbance of 0.021±0.002, the pH of 8 showed an average absorbance of 0.167±0.030, and the pH of 11 showed an average absorbance of 0.088±0.001. Based on the standard deviation, there was a lack of variability between each trial for the pH levels of 5 and 11, yet the trials of the pH of 8 varied greatly compared to the other…

Gluco p-nitrophenyl turns yellow when it is exposed to the stop solution. The absorbance of the solution that contains the Gluco p-nitrophenyl can be measured using a spectrometer. The purpose of the experiment conducted was to compare the rate of…

lake water was filtered through the fuel and then filter paper was placed into a test tube to which acetone was added. This test tube was placed in a vortex and the filter paper removed before being placed in the spectrophotometer to measure the absorbance level. Another test tube was filled with the lake water, and in this tube we added a nitrate reagent, shook the test tube, and allowed the tube to incubate for 10 minutes. The solution turned red, showing the presence of nitrate. The nitrate…

placed in a spectrophotometer test tube. The entire extracted sample along with the funnel and filter were added into the spectrophotometer test tube. The spectrophotometer was calibrated using an acetone blank and set at a wavelength of 430nm. The absorbance was then measured to determine the chlorophyll levels within the water sample. After the measurement was taken, all materials were disposed of in their respective marked disposal…

The concentration of CDCL and CSER is obtained by solving equation (1) and (2) CX = A1 / ax1 ………………..... (1) CY = [A2 – (ax2 × CX)] / ay2…...(2) Where,  A1 and A2are absorbance of mixture at λ1 and λ2respectively.  ax1 and ax2 are absorptivities of DCL at λ1 and λ2, respectively,  ay1 and ay2 are absorptivities of SER at λ1 and λ2, respectively,  CXandCYare concentrations of DCL and SER, respectively. Concentration of DCL and SER in the powder mixture is found by using equation (3) and (4)…

spectrophotometer to measure the absorbance of every component in the blank except the substrate, creating a 100% transmittance. This allow the spectrophotometer to read everything that was not in the blank when the actual sample is inserted in because it would “blank” out all the components in the “blank” solution and only read the “uncommon” component. With the active enzyme, the spectrophotometer would “blank” out the enzyme and only read the substrate absorbance because according to the lab…

As a result, lactose is a competitive inhibitor. In the presence of the inhibitor, the slope of the Lineweaver-Burk plot becomes the equation below. Using equation 7 along with the Km and the Vmax from series A, the Ki of Series B and C were calculated. The concentration of inhibitor for 50mM lactose was found to be 35714 μM using equation 1. Similar to the substrate concentration, the inhibitor concentration can be found using the same equation, but substituting in the inhibitor molar…

nm instead of 400 nm would increase the absorption numbers due to more molecules in the path of the light. Additionally, this could scatter the light and raise the numbers even more. Equation 2 shows this to be true; as ι increases, so would the absorbance. These would end up with a steeper (larger) slope on the regression…