Absorbance

The average absorbance rate in figure 2 presents the different levels of reaction in terms of the chemical compounds. Comparing the average values (Team 1 representing our results) there is not much of a pattern when viewing the absorbance for each specific test tube for each team. Although, there are some values that were around the same range including test tube 2. As for enzyme change, the high amount of enzymes increases the absorbance that shows an increase in reaction…

Thus, there should be a relationship between the concentration of the compound being studied and its absorbance. This relationship is best determined using a wavelength of light in a region of the visible spectrum where the maximum absorbance is observed. This wavelength is known as λmax and is most sensitive to the changes in concentration. The purpose of this experiment was to study the absorbance of CuSO4. Because copper compounds tend to be blue in color, 3 it was hypothesized that the CuSO4…

measurements between two different salt concentrations representing their absorbance versus time. Both figures one and two have six trend-lines that are all deceleration curves. In figure one, at the initial time of 3minutes, the six data points are relatively close to each other and are overlapping. In figure one some of data values are overlapping;…

The prediction for this experiment was: when the temperature hits 80 degrees Celsius, the catechol will be at its optimal absorbance. The alternate hypothesis tested for this following experiment was that there is a significant relationship between catechol and pH. In contrast, the null hypothesis states that there is no significant relationship between catechol and oxidase…

centrifugation and used the homogenization as the supplement for all parts of the experiment (Shultz, 2006). Concentration of the enzyme or the substrate can increase the reaction rate so the enzyme being added helps clarify the meaning of that with the absorbance readings once the enzyme is added…

following parts consisted first of varying concentrations of the enzyme were used, while leaving the substrate concentration constant and second, varying concentrations of substrate were used, while leaving the enzyme concentrations constant to gather absorbance values. The data were then graphed in the following manner: the enzyme concentrations were plotted against time in one-minute intervals over the course of 10 minutes. The rate of reactions (velocities) and enzyme activities were…

filter the precipitate more than once and still ending up with a low percentage yield and high error calculation. However, the contamination of a precipitate does not significantly alter the results for colourimetic analysis, as evident from the absorbance and concentration calculated for colourimetry in task two. Additionally, when using gravimetric analysis for larger volumes of reactants, the precipitate may be prone to become significantly…

Green actually had a negative change of -5.33%. Blue had change of 6.25% in absorbance. Dark had no change at all. Discussion The group established that the hypothesis for Experiment #1 was P2 would have the most intact chloroplasts prior to the past knowledge from our lab. Our hypothesis was right but the experiment definitely…

and to 0.5 mL of each extract solution (1 mg/mL) in screw cap test tubes. After 3 min, 1 mL of 2% Na2CO3 was then added to each test-tube and the mixture was vigorously shaken for 2 minutes and left to stand for 2 hours at room temperature. The absorbance of the supernatant solution was determined at 725 nm using 90% aqueous methanol as a solvent blank. A gallic acid standard curve was prepared and the equation derived by linear regression (y =36.84 x+0.1069) was used to determine the TPC of…

were determined and recorded in Table 4. As for the BCA assay, 100 microliters of each ovalbumin dilution were combined with 900 microliters of BCA, and the resulting solution were analyzed with a spectrophotometer in order to determine the uv absorbances at 560 nanometers (Data displayed in Table…