Absorbance

the final concentrations of dopa using the C1V1=C2V2 formula for the control groups (test tubes 1,3,5,7) which was 0mM and the experimental groups (test tubes 2,4,6,8) which was 4mM (Table 2). A standard curve of dopachrome concentrations vs. absorbance at 475nm was generated…

A. fischeriThe overall purpose of this study was to create a genomic library of Aliivibrio fischeri (A. fischeri) thus aiding in creating a restriction map of the lux operon. It also employs typical molecular techniques important for biologists to understand. In this portion of the lab, the chromosomal DNA (chDNA) will be isolated. Its purity will be measured using spectrophotometric analysis. Lastly, the DNA will be digested and verified via gel electrophoresis. A. fischeri is a gram…

time period did not permit the maximum water absorbance to be reached (i.e. a plateau of the graph). Therefore, the course of this experiment was carried out over 160 minutes, to observe the decrease in the rate of hydrolysis. Different concentrated NaCl solutions were used as the solutions that the chickpeas were soaked in, altering the maximum amount of water absorbed. As shown in Figure 1, the more dilute the NaCl solution is, the more water absorbance was taking place. Also, this trend can…

reaction order of 2. Manipulations were based on the usage of initial concentrations to calculation the rate constant for the reactions A-D with those values the average rate constant was calculated to be 2.0 x 10-5 M-1s-1. Furthermore, the initial absorbance reading of Crystal Violet in trial 1 and 2 was 1.090 and 1.100, respectively and the reaction order with respect to crystal violet was first order. It was determined to be first order based on the rationale that the correlation coefficient…

Introduction The ferric chloride test is used to determine the presence of phenols in a given sample or compound (for instance natural phenols in a plant extract). Enols, hydroxamic acids, oximes, and sulfinic acids give positive results as well. The ferric chloride test can be used to detect metabolites in urine in case of inborn error of metabolism such as phenylketonuria. In addition,the Ferric Chloride test can be used to detect salicylates in urine after an aspirin overdose.1 Background…

Experiment 3 1.the experimenter compared the data with table 1 using Excel. Results: experiment 2:  worked example of determining sample C’s GFR using the absorbance data:  Experiment3: Conclusion: Overall, the data and the results experimenter obtained are fairly accurate, since the error bar is not overlapped and small. There is no data too wild to be excluded as outliners in these experiments. However…

was failure to wipe off the outside of the test tube before placing it in the spectrophotometer. Failure to do this would make all of the absorbance levels be higher than they actually are. This error could be especially detrimental when measuring the absorbance of the 4°C peroxidase solution. The condensation on the outside of the tube would make the absorbance seem abnormally high and leveled. This could make researches believe that this temperature is more optimal than other…

spectrophometer to measure the absorbance of each of the solutions for the proteins, starchs, and the supplements. We had to adjust the nm of the spectrophometer for the starch and one group of the supplements to 580 mn.The proteins and the supplements were measured at 565 nm. A spectrophotometer is used for the measurement of reflectance of solutions. We had to blank each test tube before we put each solution into the spectrophotometer. We recorded the absorbance of each solution and put the…

from pyruvate and NADH, during the last step of anaerobic glycolysis. The reaction is also catalysed in the other direction by LDH during the Cori cycle. This reaction can be assayed using spectrophotometric techniques; peak absorbance of NADH is at 340nm, but the peak absorbance of NAD+ is 259nm (Powers, et al., 2007). Enzyme assays are performed to measure the rate of an enzyme-catalysed reaction, known as the enzyme activity. Many factors can affect the enzyme activity (including…

Homogenates of liver were provided. The homogenates were made by homogenizing liver in a 1:20 w/v (1g: 20 ml) ratio in a sucrose-phosphate buffer, in which 250mM of sucrose and 50mM NaPO, with a pH of 7.4 were added. This information was used to conduct an experiment to determine the concentration of DNA, protein, and carbohydrates in different homogenate samples, including heart, liver, and kidney of Bos taurus, while also looking for the diverse protein sizes found in each homogenate sample.…