Introduction:
The ability of substances within a solution to absorb light is a wonderful phenomenon as it aids in processes such as determining the concentration of proteins. The way that a solution absorbs light is due to the photoelectric effect. The way the photoelectric effect works is that when light comes in contact with the sample, the photons of light will react with its electrons. As the light travels through the solution, "a photon of light may interact with an electron ... [where] the photon of light transfers all of its energy to the electron" (Papagapiou, 2016). Due to this process, creations such as solar panels and solar powered satellites are able to exist (Gagnoon et al).
A spectrophotometer can be used to find the absorbency …show more content…
After determining the absorbency of the solution, the Beer-Lambert law can be used to calculate any unknown concentration. This is because the law states that "there is a direct correlation between the concentration of absorbing molecules, the distance the light travels, and the degree to which the molecules absorb light" (Papagapiou, 2016). The relation as mention can be seen through the Beer-Lambert equation:
A = Ɛ x b x c where (A) represents absorbance, (Ɛ) is the Molar absorptivity that is measured in M-1 cm-1, b symbolizes the distance the light travels, and c stands for the concentration. Based on these principles and processes, I hypothesize that the concentration of a substance and its absorbency are proportional in a sense that whenever the concentration increases, so will the absorbance of the …show more content…
One explanation would be that knowing the concentration would aid in their research if they plan on comparing results of a protein to another or from one experiment to the next. There are many ways that we can discover the quantitative value of proteins through processes such as the Bradford assay and the ultraviolet absorption process. The spectrophotometer is a common device used in many of these processes making it a vital for experiments about proteins therefore we will focus on the function of a spectrophotometer and how it is used to determine the protein
The ability of substances within a solution to absorb light is a wonderful phenomenon as it aids in processes such as determining the concentration of proteins. The way that a solution absorbs light is due to the photoelectric effect. The way the photoelectric effect works is that when light comes in contact with the sample, the photons of light will react with its electrons. As the light travels through the solution, "a photon of light may interact with an electron ... [where] the photon of light transfers all of its energy to the electron" (Papagapiou, 2016). Due to this process, creations such as solar panels and solar powered satellites are able to exist (Gagnoon et al).
A spectrophotometer can be used to find the absorbency …show more content…
After determining the absorbency of the solution, the Beer-Lambert law can be used to calculate any unknown concentration. This is because the law states that "there is a direct correlation between the concentration of absorbing molecules, the distance the light travels, and the degree to which the molecules absorb light" (Papagapiou, 2016). The relation as mention can be seen through the Beer-Lambert equation:
A = Ɛ x b x c where (A) represents absorbance, (Ɛ) is the Molar absorptivity that is measured in M-1 cm-1, b symbolizes the distance the light travels, and c stands for the concentration. Based on these principles and processes, I hypothesize that the concentration of a substance and its absorbency are proportional in a sense that whenever the concentration increases, so will the absorbance of the …show more content…
One explanation would be that knowing the concentration would aid in their research if they plan on comparing results of a protein to another or from one experiment to the next. There are many ways that we can discover the quantitative value of proteins through processes such as the Bradford assay and the ultraviolet absorption process. The spectrophotometer is a common device used in many of these processes making it a vital for experiments about proteins therefore we will focus on the function of a spectrophotometer and how it is used to determine the protein