Absorbance

levels in diabetics are much higher than the average person due to the lack of sensitivity that they have to insulin. In this experiment, we tested the hypothesis that people who are diabetic will have a higher blood glucose concentration and light absorbance than non-diabetics as tested by a spectrometer test. The absorbency rate was determined by a spectrometer test which was set to a wave length of 504nm. Blood samples were taken over time periods of 0,30,60,90 and 120 minutes. The rate of…

mg/ml were added into their own sample of the 3 ml Bradford Assay, 30 µl of water was added to a separate sample of Bradford Assay and 30 µl of the dilute liver homogenate was added to its own sample of Bradford Assay and left for 3 minutes. The absorbance of the standards and the diluted homogenate samples were recorded to create a standard curve. From this equation, the protein concentration of my sample was determined. Class averages were also obtained (Clendening). Y= 0.52x + 0.186 ,…

effect on photosynthetic activity. An experimental hypothesis was suggested that if there is an effect, in the samples which contain chloroplasts in light, the absorbance at 600 nm will decrease which means active photosynthesis will occur; in the samples which contain chloroplasts in dark and the samples without chloroplasts in light, the absorbance as 600 nm will maintain…

Such interferences in patient specimens increase the background absorbance in the reaction which is not alleviated by subtracting the secondary absorbance reading at 700 nm. For the Cobas c501 module, this results in an increased reagent background absorbance to a level which exceeds the maximum absorbance specified for the instrument detector (32000 ABS), once reagent 3 (NADPH) is added to the reaction (Figure 1). In 2014, Roche released…

Determination of Most Advantageous Sequestering Agent By: Kayla Jeeter and Ashley Driscoll Chemistry 112-508 Abstract The creation and execution of this project served to determine what material is a better sequestering agent of the polycyclic aromatic hydrocarbons (PAHs) that are found in Texas’ water. Charcoal is currently the most typical agent used to sequester PAHs. In this experiment, two different Bentonite clays (zeolites) were synthesized, one magnetized and one not, to determine if…

and a stem. The second test indicated that the green portion of a leaf did not absorb values of 510 nm though 640 nm, which are green wavelengths. Since plants reflect the colors they do not absorb, this data makes sense. It does not have a high absorbance value, meaning that it does not absorb those wavelengths. The second test also indicated the stem portion of the beet (red) did not absorb values between 510 nm through 640 as well as values between 670 and 740 nm. The second set of values are…

method, where zinc standards were prepared and from the absorbance of those standards a calibration curve was plotted, which in turn was used to determine the amount of zinc in vitamin water. The second method was standard addition. For this method, an un-spiked and spiked solution were prepared. The un-spiked solution only contained the vitamin water, while the spiked contained the vitamin water and a standard zinc spike. From the absorbance of those solutions, the amount of zinc in vitamin…

viable cells to study. It is also important to know the temperature that will result in the fastest growth, in order to get the most visible growth (4). Cell density is the number of cells found in one area. Optical density (O.D.) is the same as absorbance, and it is used for measuring bacterial growth, which would be the number of cells…

this indicates a very strong correlation between the concentration of melamine and the absorbance ratio. Hence, melamine indeed causes Au nanoparticles to aggregate and causes their Plasmon peaks to shift and the absorbance ratio to change consequently. Plasmon peaks shift to longer wavelengths indicate the nanoparticles are larger in size. This shift can be seen from the trend in absorbance in table 3; the absorbance at 520 nm decreases with the increase of melamine concentration, while it…

present in the low-calorie Gatorade, a small sample of the Gatorade was inserted into a UV-Vis spectrometer to examine which color was being absorbed and reflected. There were two peaks, where the two colors were being the most absorbed. Once the absorbance was figured out, the food dyes that is needed in the Gatorade was present by using the color spectrum and the wavelength. These colors were green (514 nm) and orange (629.6 nm). Then the emitting colors can be solved. The colors that were…