DIAGNOSIS OF ANAEMIA: DETERMINATION OF HAEMOGLOBIN CONCENTRATION
Introduction
Red blood cells contain the protein molecule haemoglobin (Hb); it is made up of four polypeptide chains and contains iron. Its function is to carry oxygen from the lungs to the bodies tissue and to carry carbon dioxide from tissue back to the lungs. Anaemia can be caused when there is a lack of iron in the body which leads to a reduction in the number of red blood cells. Iron is used to carry oxygen in the blood so If you have fewer red blood cells than is normal, your organs and tissues won 't get as much oxygen and this can be damaging (NHS Choices, 2016). Oxygen is used in respiration which provides energy in the form of ATP for things like metabolic reactions …show more content…
A series of dilutions were prepared by mixing the stock solution was water in a test tube. The concentrations used were 0.139 mg/mL, 0.111 mg/mL, 0.083mg/mL, 0.057mg/mL and 0.028 mg/mL. There were two lots of each concentration made. The spectrophotometer was set at the previously calculate peak wavelength of 409mn and zeroed with deionised water. A cuvette was filled with 0.139 mg/mL and the absorbance was measured, then again with the same concentration from the other test tube and an average was calculated. This was repeated for each concentration. Then a graph was plotted with concentration on the x-axis and the absorbance along the …show more content…
4 shows the absorbance of different concentrations of Hb at 409nm. To calculate the concentration of the solution I used the equation: Final concentration= (stock added (ml) x stock concentration) / total volume (ml). for example, x= (4x0.139)/5 x=0.111
A graph to show the absorbance of different concentrations of Hb at wavelength 409nm
A table to show the absorbance at 409nm wavelength of the blood sample from the female patient.
Discussion
Fig. 1 shows the absorbance of light of Hb at varying wavelengths, from 375nm to 415nm. The table shows the absorbance increases as the wavelength increase, up to 410nm where the absorbance was 0.868. This shows that the peak absorption is close to this wavelength. Fig. 2 shows that the spectrophotometer was increased by 1nm intervals to get an exact reading where the light is absorbed the most, this wavelength can then be used to determine the concentration of Hb in the unknown sample. The highest absorbance was 0.877 at 409nm. Fig. 3 shows this information in a simple and easy to read
Introduction
Red blood cells contain the protein molecule haemoglobin (Hb); it is made up of four polypeptide chains and contains iron. Its function is to carry oxygen from the lungs to the bodies tissue and to carry carbon dioxide from tissue back to the lungs. Anaemia can be caused when there is a lack of iron in the body which leads to a reduction in the number of red blood cells. Iron is used to carry oxygen in the blood so If you have fewer red blood cells than is normal, your organs and tissues won 't get as much oxygen and this can be damaging (NHS Choices, 2016). Oxygen is used in respiration which provides energy in the form of ATP for things like metabolic reactions …show more content…
A series of dilutions were prepared by mixing the stock solution was water in a test tube. The concentrations used were 0.139 mg/mL, 0.111 mg/mL, 0.083mg/mL, 0.057mg/mL and 0.028 mg/mL. There were two lots of each concentration made. The spectrophotometer was set at the previously calculate peak wavelength of 409mn and zeroed with deionised water. A cuvette was filled with 0.139 mg/mL and the absorbance was measured, then again with the same concentration from the other test tube and an average was calculated. This was repeated for each concentration. Then a graph was plotted with concentration on the x-axis and the absorbance along the …show more content…
4 shows the absorbance of different concentrations of Hb at 409nm. To calculate the concentration of the solution I used the equation: Final concentration= (stock added (ml) x stock concentration) / total volume (ml). for example, x= (4x0.139)/5 x=0.111
A graph to show the absorbance of different concentrations of Hb at wavelength 409nm
A table to show the absorbance at 409nm wavelength of the blood sample from the female patient.
Discussion
Fig. 1 shows the absorbance of light of Hb at varying wavelengths, from 375nm to 415nm. The table shows the absorbance increases as the wavelength increase, up to 410nm where the absorbance was 0.868. This shows that the peak absorption is close to this wavelength. Fig. 2 shows that the spectrophotometer was increased by 1nm intervals to get an exact reading where the light is absorbed the most, this wavelength can then be used to determine the concentration of Hb in the unknown sample. The highest absorbance was 0.877 at 409nm. Fig. 3 shows this information in a simple and easy to read