Catechol Enzyme Lab Report

753 Words 4 Pages
Discussion

In this study, the Catechol enzyme was studied under the conditions of varying pH, temperature, substrate concentration, and enzyme concentration. In Figure 1, the data suggested that the trend was neither directly nor inversely proportional, but the highest activity rate was at 24°C. Most enzymes denatured at higher temperatures of approximately 40°C, which led to the inability to see any color change (Helms et al., 1998). At lower temperatures, the enzyme was somewhat efficient because molecules move slower at lower temperatures, so enzymes lost productivity. Figure 2 agrees with the introductory statement concerning that there is a specific pH which was optimal for enzymes due to a stable and preferred ion concentration. When
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However, when the substrate concentration was increased, the intensity of color stopped increasing due to the enzymes being unable to to process at a faster rate. All of the alternate hypothesizes were supported; in the experiments, section one (with Figure 1) had an optimal temperature at approximately 24°C; section two (with Figure 2) had an optimal pH at approximately pH8; in section 3 (with Figure 3) the color continued to increase with the addition of more enzymes; and in section 4 (with Figure 4) the color continued to increase until it reached a maximum velocity with the addition of more substrates. One desired retrial for the experiment would be to test for more points after 20 drops of potato juice in Figure 3. Understanding the effects of how these properties alter the ability of the catecholase enzyme could allow for better insight into decreasing time needed for biochemical reactions, proper food storage, and the condition of …show more content…
As enzyme concentration increased, the color strength continued to increase. In Biology in the Laboratory, this is confirmed by the section concerning the effect enzyme has on activity (Helms et al,. 1998).

In Figure 4, the substrate and color relation had a direct relation to an extent. As substrate concentration increased, the color strength continued to increase until the solution reached maximum velocity. In Biology in the Laboratory, this is confirmed by the section concerning the effect substrate concentration has on activity (Helms et al,. 1998).

Conclusion
1. For the catechol enzyme, the optimal pH is 8 and temperature desired in order to function effectively is near near room temperature.
2. When catechol is in excess and substrate is increased, the rate of reaction increases to a maximum limit due to the inability of the enzyme to work higher than its normal rate of reaction.
3. When the catechol is the substrate in excess and the enzyme increased, the rate of reaction increases exponentially due to the increasing amount of enzymes able to catabolize more

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