Absorbance

minute increments. The amount of reduction of DCIP as measured by the decreased absorbance by the Spec 20 over time can be viewed in Figure 1. The total change in absorbance can be seen in Figure 2. The line representing no cell fraction was a negative control in which little to no change was expected. The independent variable was the cell fraction added to solution, and the dependent fraction was the resulting absorbance decrease. The solution with supernatant 3 has the smallest change in…

peroxidase, which was extracted from the organism Brassica rapa. The peroxidase was standardized at 0.5ml, 1.0ml, and 2.0ml to determine the amount of extract needed for testing, which was one milliliter. The spectrophotometer was used to measure the absorbance in the guaiacol dye to determine the rate of reaction. The…

Introduction: The purpose of this lab was to use the absorbance of light through a solution to determine the concentration of Blue Dye 1 in Cool Blue Gatorade. In today's society most people are health conscious and food coloring and dyes have been a controversial topic. Blue Dye 1 is one of the most popular FDA-approved dyes used in food such as fruits, sauces, and baking mixes. Such dyes are not limited to foods but also used in shampoos, detergents, and vitamins just to name a few (Hennessey)…

lab was to observe the amount of light a solution absorbs, and use the relationships in the Beer-Lambert Law to determine the quantity of a certain compound within a complex sample. First, the relationships between wavelength, concentration, and absorbance were determined. This lab focused on identifying the absorption of copper in various brass samples, and finding the percent copper in brass. In the first activity of the lab, the scientist was trying to determine the difference in molar…

then makes the overall reaction second order. Maximum absorbance turned out to be 1.393 at 630 nanometers. Molar concentration was found to be 1.01 X 10-5 and Kobs from the graph was negative .293 while the rate constant was calculated to be 7.58 X 105. Wavelength of maximum absorbance was found by repeated testing of wavelengths until the max absorbance was found to be 1.393 at 630 nanometers. Using the Beers-Lambert law, maximum absorbance and the molar absorptivity of the blue dye (1.38 X…

and set to maximum absorbance wavelength. Ten test tubes were then prepared. The Spec 20 was set to 0% and a test tube of blank solution (water) was wiped off and placed into the Spec 20. Transmittance was then set to 100% to calibrate the machine. Next, five test tubes were prepared and labeled with one of the standard solutions of 0.05 M, 0.1 M, 0.2 M, and 0.5 M CuSO4 in them. The Spec 20 was set to 600 nm and % transmittance was measured of the standard solutions. Absorbance was calculated…

1.0 Introduction 4-hydroxy-3methbenzaldehyde is the scientific name for Vanillin. What is vanillin? It is a substance that adds flavor to the special taste and smell in food. It is extracted from high quality beans (1). A specific solvent named ethanol can be used to extract vanillin. There are two types of vanillin namely natural vanillin and artificial vanillin. There is artificial vanillin in the market because natural vanillin is costly. Before a chemical experiment or process is carried out…

Monica Gromala Section # 003 BIOL 130L (B2 149) Tuesday (9:30am -12:20pm) Date of Experiment: 21/10/2014 INTRODUCTION The purpose of spectroscopy is to observe the relationship between substances in a solvent, and the associated absorbance and transmittance through a range of concentrations (Lothian & Twyman, 1969). However, one must understand the underlying principles of the electromagnetic spectrum in order to analyze the results of the techniques used in this experiment.…

The conversion is necessary so that all recorded data would have the same units for comparison and is needed due to the fact that the basic unit for concentration is mg/ml. Table 3: Calculated Protein Concentrations Sample Initial Absorbance (OD) Dilution Absorbance (1:50) with DI water Calculated Concentration with dilution in consideration(mg/ml) Concentration on Label (mg/ml) Protein Shake 1.912 0.164 13.667 21.1 Whole Milk 2.391 0.431 35.914 33.8 Muscle Milk 2.176 0.700 58.333…

The higher the concentration of polyphenol-oxidase, would lead to higher amounts of molecules in the solution, which can absorb more photons resulting to higher absorbance values. All deceleration curves except for our control variable are gradually increasing as time is passing by and levels off reaching equilibrium. A molecular explanation for the results in figure 1 is that polyphenol oxidase is a catalyst that…