RNA splicing

(DIG) labeled single strand cRNA probes. The DIG-labeled cRNA probes were transcribed in vitro from cDNAs of rat GR (~0.4 kb) and rat SERT (~0.7 kb) in pGEM-3Zf vectors with T7 RNA polymerase and DIG RNA labeling mix (Roche, Branford, CT, USA). The amount of the transcript was estimated by comparison to DIG-labeled control RNA (Roche, Branford, CT, USA) in Dot-blot assays on nylon membranes (Roche, Branford, CT, USA). The ISH protocol is similar to the one from Cold Spring Harbor Laboratory with…

central dogma, first devised by Francis Crick, is a classical backbone of living cells to essentially perform the processes from cell division to death through the DNA, RNA, and protein information ways. More specifically, the central dogma defines the transfer of sequence information during DNA replication, transcription into RNA, and translation into amino-acid chains forming proteins. It also states that information cannot flow from protein to protein or nucleic acid. In addition, Hirao &…

Transcription is a process which information from DNA is converted into its RNA equivalent which also refers to the synthesis of RNA copy of information encoded on DNA. The same principles of transcriptional regulation apply to both eukaryotes and prokaryotes. Transcription involves in access of transcription apparatus to DNA, recognition of promoter sequences, initiation of RNA synthesis, elongation of RNA, and termination. Transcriptional in eukaryotes is more complex compared to prokaryotes…

Transcription is the process in which DNA is converted to RNA. Ribonucleic Acid (RNA) is a polymeric molecule. RNA is involved in protein synthesis, and it is sometimes even in the transmission of genetic information. The main job of RNA is to transfer genetic information that is needed for the creation of proteins from the nucleus to the ribosome. Without RNA proteins could never be made (News-Medical). Transcription is the process of making an RNA copy of gene sequences. This particular copy…

dogma of molecular biology? The central dogma is the explanation of how genetic information flows as it is passed from DNA to RNA to protein. DNA replicates and converts the information it carries into RNA, this process is called transcription. During transcription the information is passed through nucleic acids and there is no need for a change in the “language.” When RNA continues the flow of information and it is passed to proteins, this is called translation. It is called translation…

phenotypes of the individuals. The DNA is then copied by the RNA and synthesized into protein. The process of transcription, which is the synthesis of RNA from a DNA template, is where the instruction of the gene expression is most likely to occur. Prokaryote allows a continual synthesis of protein to occur. On the other hand, in…

Theory, RNA World, etc.). Life chemically first arose from inorganic matter due to the environment created by hydrothermal vents, the production of RNA, and clay hydrogels. Primarily, the environment created by hydrothermal vents could have led to…

about because RNA was formed. This occurred because organic molecules on Earth were able to form ribonucleotides from the assistance of a clay catalyst. Eventually, these ribonucleotides formed RNA, which, through time, began to fold and replicate itself. As replication got faster, RNA's started to attract amino acids and hold them together. Bonds started forming, and proteins were able to be produced. As more years passed, a lipid membrane enclosed the structure to protect the RNA and proteins,…

Proposed research: Aims and Objectives: Aim1: To address the roles of the Hira complex in rRNA transcription and embryonic development Aim2: To characterise rDNA chromatin organisation in the oocyte Aim3: To elucidate the transcriptional machinery of RNA Polymerase I in the oocyte Experimental Approach: Aim1: To address the roles of the Hira complex in rRNA transcription and embryonic development The Hira histone chaperone complex is an evolutionarily conserved complex, which is composed of…

While this may be the case, the antibody (IgM) cross- reactivity with other flaviviruses can be a problem when trying to come up with a precise diagnosis that is specific to Zika virus. In most cases, many tests often confirm the presence of the virus RNA in the blood of the patient or any other body fluids such as semen, saliva, or…