RNA splicing

These effects of stretching prominently appear to affect stretch tolerance with no changes in the viscoelastic properties of the muscle (Halbertsma & Göeken, 1994) (Magnusson, Simonsen, Aagaard, Sørensen, & Kjaer, 1996). The mechanism for increased performance following chronic stretching is believed to be related to stretch-induced hypertrophy, which is discussed more when examining the adaption mechanisms. Adaptation Mechanisms The adaptation mechanisms of acute and chronic stretching…

Introduction Hutchinson Gilford Progeria Syndrome (HGPS) is a fatal disease that is characterized by the appearance of rapid aging in children. HGPS is rare, affecting roughly 1 in 8 million live births. HGPS can be identified by early symptoms that include slow development, limited growth, alopecia, localized scleroderma, and a distinct facial structure which includes a small face, shallow jaw, and pinched nose. As the disease progresses, new symptoms arise, such as hearing and vision loss…

The biological instructions that make each species unique is a code often referred to as Deoxyribonucleic acid or DNA. In every cell in an organism DNA can be found in the cell’s nucleus. mRNA a replication of DNA leaves the cell’s nucleus and journeys to the mitochondria. Adenine (A), Guanine (G), Cytosine (C) and Thymine (T) are the four coded chemical bases that the DNA uses to store information. Three of these bases code for a triplet of a codon. DNA’s four chemical bases each have a pair; A…

Designer babies are the new way to bioengineer offspring, to change the different genetics of a child. Scientists will be able to eventually use transcription and translation to change the different traits like; gender, appearance, intelligence, diseases, and personality. There is much controversy on whether designer babies should exist because of ethical controversy and designer babies causing additional racism in our country. Only the wealthy will be able to have designer babies, and will…

According to The General Biology I Lab manual, involved in Gene expression are two complex cellular pathways, each being subjected to regulation (Alberte et al., 2012). The first pathway is the synthesis of an RNA molecule from a DNA strand. This process is called transcription. When the RNA molecule exits the nucleus, it can be translated into a polypeptide sequence. This process is called translation. With some adjustments, these pathways happen in both prokaryotes and eukaryotes. Plasmids…

expressed in a given sample. While the RNA from the unaffected individuals produces the expected product, the diagram shows an abnormal product of 489 bp in the samples that were affected by HGPS due to the activation of the cryptic splice site. A western blot, a procedure used to identify specific amino acid sequences in proteins, was performed (section d) using an antibody against lamin A/C. This was conducted to determine whether the mutant messenger RNA was actually translated into a…

An isotope is an atom containing different numbers of neutrons, differing in atomic mass. Basically an atom with a different atomic mass. What’s special about radioactive isotopes is that a radioactive isotope has a nucleus that decays spontaneously giving off particles and energy. 6. Radioactive tracers are used in science to label certain chemical substances, so metabolic processes can be followed and substances can be located in an organism. Radioactive tracers are important diagnostic…

uses different buffers to optimize the lysis procedure making sure just DNA is absorbed while undesired products are not retained and in opposite are found in the flow-through. These buffers are: P1 (a resuspension buffer that hydrolyzes not DNA but RNA since it contains the RNase enzyme), P2 (a lysis buffer, it opens, breaks or lyse the cell wall and contains SDS and NaOH), and N3 (a neutralization buffer that contains an acetate buffer which helps in the neutralization of NaOH and guanidine…

1) Alcaligenes faecalis is a Gram-negative coccobacilli that is motile, oxidase positive, catalase positive, non-spore-forming, non-fermenting, slow growing, and citrate positive obligate aerobe (Mahon et al., 2014) (Sachdeva et al., 1963). Its colony morphology is flat, thin, and rough with irregular edging (Mahon et al., 2014) and the flagella varies between the usual shape versus the curly structure, however, both shapes do not exist on the same individual bacteria (Leifson, 1960). The…

are essential for drug-resistance. Computationally inferred drug combinations will be validated for their effect on MRs’ activity and ability to govern drug resistance and tumorigenicity. Furthermore, inferred drug combinations will be analyzed using RNA sequencing of treated tumors for their synergistic action and potential benefits for patients with castration-resistant prostate cancer. Approach…