Microscope slide

water under the microscope. Taking note of their size, movements, and characteristics to classify the different types of protists from applying previous knowledge of protists. Materials: The materials in this lab that will be needed are a microscope, slide, coverslips, a pipette, and a dish of pond water. Procedure: Prepare a slide by using the pipette to apply two drops of pond water on a slide and properly place a coverslip. Place the prepared slide under the microscope to view and…

There was an extra control slide so that the microscope could be adjusted without harming the samples. The cover slip was placed on top and nail polish was added to the sides of the cover slip to prevent the sample from drying out. With the 40x lens of the Zeiss Axiovision Widefield Fluorescence Microscope, images of the cells were captured. The two channels that were used were DAPI and DIC. DAPI had an exposure time…

For the purpose of this assignment, I researched an article titled, “The Role of DJ-1 in the Pathogenesis of Endometriosis” by Priyanka Rai and Sisinthy Shivajj. The article discusses many components in which are relevant to the disease, endometriosis. Endometriosis is a disease among women in which causes infertility and severe pelvic pain along with other health concerns. It is seen to occur within 10% of woman whom are of a reproductive or sexually-active age. It is due to the occurrence…

Purpose: Observe a compound light microscope to learn the names of its major parts. Research: Microscope is an essential tool for the study of small structures such as cells. The most common type of microscope is the bright-field microscope, used for this purpose is the compound light microscope, it is called compound because it uses two sets of lenses the objective and the ocular. The ocular lens usually magnifies 10x.…

It can be explained as follows; the ultrafast electron pulses with a duration of couple hundreds of femtosecond are generated by the photoemission process by illuminating the photocathode inside the microscope by UV laser pulses. These electron pulses are accelerated inside the microscope and incident on the sample under study together with visible laser pulses. The photon-electron coupling between the optical pulses and the electron pulses takes place when the energy-momentum conservation…

1. How are the photographs taken with a 3D microscope different than those taken with a regular camera? How are they similar? The 3D microscope photographs are used to show detail that a regular photograph could not, for example at a crime scene. The similarity is the basic image, it is the same image but the only difference it has with a 3D microscope is the detail shown. 2. How can photography allow us to view the world around us in different ways? Photography captures not a second of the…

Then, observations were made of the mCherry fluorescence under a fluorescent microscope with a “Flur 20X” objective and FIT-C Filter Set, which produced green light and made the specimen’s basal body formation glow red (shown in Fig. 6). Each strain of fixed cells was prepared on a separate wet mount slide, and the observations made were focused mainly of the shape and intensity of fluorescence coming from the oral groove location on the Tetrahymena…

Cellular Theory & Microscopes Robert Hooke Robert Hooke was an English scientist credited most famously for the creation of Hooke’s Law (a principle of physics that states that the force needed to extend or compress a spring by some distance is proportional to that distance) in 1660 and the discovery of plant cells using a compound microscope in 1663. The compound microscope uses two or more lenses, which are connected by a hollow tube. The top lens (called the eyepiece) is the one that people…

A 12-well tissue culture plate was used to carry out the LIVE/DEAD Assay. Well “A” contained the untreated HepG2 cells. Well “B” contained camptothecin, which is used as a control for killing the HepG2 cells instead of the use of plumbagin. Well “C” contained the compound Epigallocatechin Gallate. Well “D” remained empty, because a vehicle treatment is not needed for this experiment. Each well contained 1 ml of HepG2 cells. A negative and positive control where both set up in the 12-well tissue…

An inverted fluorescence microscope is an inverted compound light microscope equipped with modules to internally separate the different wavelengths of light, and direct them to respective detection channels. Its main components include a light source, an objective, a filter cube and a detection unit, which can be an eyepiece or a camera (Fig.1). Filter cube is a component which separates a fluorescence microscope from a light microscope. It consists of one excitation filter, one emission filter…