Microscope components

Purpose: Observe a compound light microscope to learn the names of its major parts. Research: Microscope is an essential tool for the study of small structures such as cells. The most common type of microscope is the bright-field microscope, used for this purpose is the compound light microscope, it is called compound because it uses two sets of lenses the objective and the ocular. The ocular lens usually magnifies 10x. The objective lens can magnify 4x, 10x, 40x, or 100x. Hypothesis: To examine the slide of the three colored threads, and the…

Cellular Theory & Microscopes Robert Hooke Robert Hooke was an English scientist credited most famously for the creation of Hooke’s Law (a principle of physics that states that the force needed to extend or compress a spring by some distance is proportional to that distance) in 1660 and the discovery of plant cells using a compound microscope in 1663. The compound microscope uses two or more lenses, which are connected by a hollow tube. The top lens (called the eyepiece) is the one that people…

An inverted fluorescence microscope is an inverted compound light microscope equipped with modules to internally separate the different wavelengths of light, and direct them to respective detection channels. Its main components include a light source, an objective, a filter cube and a detection unit, which can be an eyepiece or a camera (Fig.1). Filter cube is a component which separates a fluorescence microscope from a light microscope. It consists of one excitation filter, one emission filter…

Observation of plant and animal cells through a light microscope. A cell is the most basic structure of any living organism and is capable of independently reproducing. Cells can be grouped into two categories, prokaryotic and eukaryotic. In a eukaryotic cell there are small organelles that carry out specific functions which can be compared to the organs in the human body. Some of these organelles include the nucleus, which holds the cells DNA, mitochondria, which produce ATP from glucose and…

For the purpose of this assignment, I researched an article titled, “The Role of DJ-1 in the Pathogenesis of Endometriosis” by Priyanka Rai and Sisinthy Shivajj. The article discusses many components in which are relevant to the disease, endometriosis. Endometriosis is a disease among women in which causes infertility and severe pelvic pain along with other health concerns. It is seen to occur within 10% of woman whom are of a reproductive or sexually-active age. It is due to the occurrence…

bacteria under the microscope with the resolutions 10x, 40x, and 100x with the use of immersion oil. Observations were noted and we replaced that yogurt slide with the prepared, stained yogurt slide. We compared and noted the observations between the two yogurt samples. Exercise 3: Preparing and Observing a Blood…

During the microscope lab, my partner and I learned how to properly use a microscope, calculate the field of view, and view and prepare slides. Microscopes are instruments used to view very small objects that cannot be seen with the naked eye. We found that microscopes have many different parts to them, including the diaphragm, ocular lens, and objective lenses. There are three powers of magnification, scanning power (4x), low power (10x), and high power (40x). To find the total magnification,…

photocathode inside the microscope by UV laser pulses. These electron pulses are accelerated inside the microscope and incident on the sample under study together with visible laser pulses. The photon-electron coupling between the optical pulses and the electron pulses takes place when the energy-momentum conservation condition is satisfied (27, 28). This inelastic interaction leads to gain/loss of photon quanta via electron packets, which can be resolved in the electron energy spectrum…

a 3D microscope different than those taken with a regular camera? How are they similar? The 3D microscope photographs are used to show detail that a regular photograph could not, for example at a crime scene. The similarity is the basic image, it is the same image but the only difference it has with a 3D microscope is the detail shown. 2. How can photography allow us to view the world around us in different ways? Photography captures not a second of the environment, but to capture a moment. We…

A light microscope works like a telescope using glass lenses to reflect light, which magnifies images which are naked to the human eye such as cells and dust. The lens closest to your eye is called the eyepiece lens. This usually has a magnification of x10. Objective lenses are lenses of several different strengths which magnify the image. The stage is the place where the microscopic slide sits. This is held in place by the clips. The light source (can be a mirror or a bulb) projects light onto…