Materials and Methods:
This study was carried out at a lab in which involved numerous steps and experimental procedures. The overexpression and reduction of expression of the DJ-1 protein was acted upon normal endometrial cells and endometriotic cells through utilization of siRNA was studied. For the purpose of this experiment, several reagents were used such as: DMEM, FCS, antibiotics, and phenol red free DMEM. There were several cells that were utilized such as: stromal cells, endometriotic epithelial cells, and immortalized human endometrial surface epithelial cells. These cells were then injected with the 200 nM DJ-1 SMARTpool siRNA, which specifically targeted the DJ-1 gene or the non-silencing control. Protein extraction and immunoblotting were then done to prepare the adhesion assay. The cells were lysed and the proteins were eluted through boiling in 3X SDS sample buffer and then the amino concentration was calculated. The proteins were placed in a nitrocellulose membrane, in which they were then ready for immunoblotting. GFP as well as other materials were used as the antibodies for conjugation. The bands were then observed. In order to do the cell