Microscopy

Transmission electron microscopy, or TEM, is a variation of low field, high-powered electron microscopy. Created by Ernest Ruska and Max Knoll of the Berlin Technische Hochschule in 1931, the invention of this novel microscope allowed for the surpassing of the visible light resolution barrier. Since its inception, the TEM has become a resolution standard in the field of imagery, and further advances have led to the progression of high quality atomic viewing. The development of the electron microscope in the 1930s revolutionized biology, allowing for organelles, such as mitochondria, to be seen in detail for the first time. Unlike light microscopy, TEM uses a beam of electrons, denoted as a cathode ray, to scan samples. While the traditional optical microscope is restricted in resolution by the wavelength of…

endometrium of women who had endometriosis and those whom didn’t have it. The results of the two populations were compared. Due to this being done, it was shown that a particular protein known as DJ-1, is present and upregulated in eutopic endometrium of the woman surveyed with endometriosis. Therefore, it was inferred, that this protein may have impact or involvement of how endometriosis occurs. DJ-1 is a protein that is known to affect cell survival and growth as well as in ant oxidative…

During the microscope lab, my partner and I learned how to properly use a microscope, calculate the field of view, and view and prepare slides. Microscopes are instruments used to view very small objects that cannot be seen with the naked eye. We found that microscopes have many different parts to them, including the diaphragm, ocular lens, and objective lenses. There are three powers of magnification, scanning power (4x), low power (10x), and high power (40x). To find the total magnification,…

During this lab, Sfr13 mutant and wild type Tetrahymena’s number of India Ink vacuoles and sizes were observed over multiple trials, and then the averages were compared to see if there was any difference between the mutant and WT. Furthermore, the two strains of Tetrahymena were stained with mCherry fluorescence, so that the oral grooves could be examined and compared under fluorescence microscopy. This experiment was designed to eliminate as much bias as possible by replicating the experiment…

This article discusses the research behind the bioluminescent millipedes. These rare species are classified as Motyxia. Paul Marek is an entomologist who was determined to find these rare millipedes near San Luis Obispo, California. Marek set out on his journey in 2013. Bioluminescent millipedes are fluorescent, meaning they glow a green hue under UV light. Marek found it easier to search for the millipedes during the night. Soon after arriving at his location he found three bioluminescent…

Introduction The microscope is an optical instrument that produces large images of small objects that cannot be observed by the human naked eye. There are two types of light microscopes: compound microscope and Electron microscope. Light microscopes are divided into two categories: Compound microscopes and Stereoscope microscopes. Electron microscope functions in the following way; a stream of electrons emitted from an electron source built in the microscope, the obtained image is in black and…

1. I believe that the compound light microscope would be the best microscope to use in order to observe very small objects or organisms on a slide. First, this microscope can magnify objects up to 1000 times. Second, this microscope is typically used to observe cell size, shape, and general appearance. The best microscope to use in order to observe internal structure, is the transmission electron microscope. The reason for this is because this particular microscope can magnify objects up to…

*** The methods based off the procedures section of the Lab Handout *** (Blanar, 2017) In this experiment, the first step performed was that a compound light microscope was obtained. A Daphnia magna specimen was then pipetted into the well of a concave slide, but not too much water was added to prevent the specimen from swimming away from focus on the slide. If too much water is present a tissue can be used to wipe off the excess. The specimen was put under the microscope at the lowest…

Microscopy is one of science’s greatest innovations. The microscope has revolutionised countless areas of scientific research and has become an icon of science itself. From Anton van Leeuwenhoek discovering micro-organisms in the 1670’s to the highly powerful atomic microscopes used today, microscopy has furthered scientific knowledge in many, many ways [1]. It has practical applications in very many areas of research such as biotechnology, materials science, microbiology, environmental science…

The slide used during the Microscope Lab was a wet-mount slide which was used in order to view the specimens from the microscope. A wet-mount is a method for preparing specimens to view using a microscope. A myriad of specimens look better when placed in a drop of water on the microscope slide. The water supports the sample and fills the space between the coverslip and the slide. This allows light to pass easily through the slide, the sample, and the coverslip. Scientists use the wet-mount…