Microscopy

differential centrifugation, microscopy, and succinate dehydrogenase assay. For the fist part of the lab density played a key role in the separation of the cells content because mitochondria and nuclei are very different in size, mitochondria is approximately ten times smaller than nuclei. So it was hypothesized that nuclei would be present in pallet 1, and mitochondrial would be the content for pallet two because the density difference between the two organelles. During the microscopy, the…

FlowMAP is a microfluidic platform developed to measure the overall hemostatic phenotype of an individual, coagulation profile. It is willing to replace the current tests of thrombogenecity, that are focused on isolated components of clotting and under standardized conditions. These individual’s hemostatic profiles under certain conditions and particular drugs will lead to a better understanding of bleeding risk ad clinical clotting prediction. Unlike other complex bench-top tests, it can…

Content.  Introduction.  Parts of light microscope.  Steps to setup light microscope.  Uses of light microscope.  How to clean light microscope.  Maintain the light microscope.  Summery.  References. Introduction. Light microscope is an instrument which used to magnify small specimen which cannot be seen through naked eye, by using a light source. It helps to magnify the small object to 1000* times than the real size of the real object. In light microscope light ray is send and…

Table 1 depicts the Velocity of DCIP being reduced by SDH. Table 2 shows a comparison between SDH assay and the images perceived from the microscopy. When looking at table 2, it was seen that Nuclei and mitochondria were only located in the filtrate. S1 contained mostly mitochondria, while P1 contained mostly mitochondria. S2 had some mitochondria while P2 had a majority of mitochondria. When…

entities. So, to understand Osmosis is to understand all living things. When a solution that has the same osmotic weight as another substance, mostly one in a cell is said to be Isotonic because the solutes inside and outside of the cell or the same (Microscopy and Microscopes Magazine 2013). They balance each other out. If concentration of a solute is less outside the cell versus inside the cell, the cell tends to be Hypotonic and when the solutes are greater outside the cell versus inside the…

in repair, regulation of DNA repair, and molecular levels in nucleus. The procedure is fairly simple and can be performed easily by placing a cell on a agrose slide, performing cell lysis, electrophoresis, neutralization, fixation, staining, and microscopy analysis. The comet assay is an important and easy technique, which can be used to determine the about of double stranded and single stranded DNA breaks in a…

real live laboratory setting and to introduce microscopic analysis. There were two objectives for this laboratory study: [1] Learn how to handle to microscope and familiarize its structural functions, and [2] examine real live specimens through microscopy. Introduction A microscope is a fundamental equipment used in observing specimens that are not visible to the naked eye due to its minuscule proportions; predominantly when observing eukaryotic and prokaryotic cells. A pathologist for…

In TSB, the WT organism formed no agglomerates, while Δagr and Δpsm developed a small number. In SF, all organisms developed large agglomerates. The data is also visualized by microscopy in Fig. 4 (B). Researchers concluded that the lack of statistical difference between the WT and mutants in SF proves that the lack of PSM is responsible for the development of exceptionally large agglomerates. Immunoblotting with PIA-specific antibodies…

and would destroy all DNA. Investigators later found help from a microscopy detective that would aid in telling them what the stomach materials were (Dowling et al.). The detective used a polarizing light microscope to analyze the stomach contents. A polarizing light microscope is a microscope that uses polarized light to analyze the optical properties and internals of specimens (“What You Ought to Know about Polarising Light Microscopy”, bitesizebio.com). Polarized light is a type of light that…

Nano-dots in Mammalian Gametes Imagining1 The Nano-dots, or Quantum Dots (QD), are nano-scale semiconductor devices with diameter up to 10nm, which were first discovered in 1980s2. Once stimulated, they give emission of light with specific colours, which is then visualised1-3. These nano particles have high size-tunability, once illuminated, different emission wavelength can be obtained by manipulating the size of dots3. Inorganic QDs are made up of two components, an atom-cluster core with…