Microscopy

measures the amount of alkaline phosphatase enzymes in the blood stream. 6. Summarize the findings in figure 5. Do you agree with the findings? Why or why not? Micrographs of osteogenic assessment of hMSCs on CHACC, by electron microscopy in vitro (A–D) and light microscopy in vivo (E), (F). (A), (C) hMSCs on CHACC, 16 days in control culture medium. (B), (D) hMSCs on CHACC, 16 days in osteogenic medium. Notice the rougher and more mature collagen fibres in (D) in comparison with (C). (E)…

explores the sort of concerns from which my cloud may have found its genesis. Specifically, Hacking explores the nature of the reliability of scientific experimentation with increasingly advanced experimental apparatuses through a discussion of microscopy, which he considers to be an oft-ignored part of scientific apparatuses so far as philosophy of science is…

Bovine serum albumin (BSA) is usually used for generation of nanoparticles (NPs) in drug delivery systems. This paper presents a study on the simple coacervation method for fabrication of BSA nanoparticles.An artificial neural network (ANN) model was developed to predict the diameter of nanoparticles based on experimental data. Effect of pH, temperature, initial BSA and cross linker concentration, molar ratio of BSA to ethanol, agitation rate and rate of ethanol addition were investigated on the…

Isolated Colonies and Soil Samples A. Purification of Serratia marcescens and Staphylococcus epidermis The objective was to grow isolated colonies on a nutrient agar medium (NA). Many microorganisms found in soil are able to grow on this medium. Since there are many organisms that are found in the soil, only a few of this organisms are adapted to live in this specific medium obtained. The NA plate is the first way to isolate microorganisms because the organisms that will be left on the plate…

Microbes of the infected root canal system Historically, the most wide-spread method for detecting and characterizing bacteria has been by culturing. Culturing bacteria allows the analysis of their morphology, virulence, gram-staining pattern, the quantization of viable microorganisms, as well as the testing of their susceptibility to antibiotics. Consequently, this method may not allow normally viable bacteria to cultivate as the selected culturing media may not have the nutritional and…

Lead and zinc are mostly present at the same occupational source, usually found as co-contaminants at specific sites and are among the top 10 substances found most frequently at national priorities list (NPL) sites with a completed exposure pathway.1 Lead has been known to humankind from ancient times and its use was increased immensely from 17th century onwards due to industrial revolution. Primarily lead was used in paints, ships, gasoline, pottery etc. However upon recognizing debilitated…

Introduction: Nano tribology is a study field of interfacial phenomenon in nano scale. The interaction between two contacting solids during a relative motion from atomic to microscale is important to understand the friction, wear, adhesion, indentation phenomenon. Now a days it has a plenty application in micro electro mechanical system (MEMS), nano electro mechanical system (NEMS), magnetic storage, lubricant manufacturing and many more field. There are few instruments in the market…

the presence of a colloidal suspension of gold nanoparticles. Gold nanoparticles (Au NPs) were synthesized by standard citrate reduction method. Au NPs and PPy-Au NCs were characterized by Transmission Electron Microscopy (TEM), Atomic Forced Microscopy (AFM), Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and X-ray diffraction (XRD) techniques for morphological and structural confirmations. The dc- electrical conductivity of PPy-Au NCs was…

Observation of the roots under light microscopy The roots of both Col and rsw1 plants of Arabidopsis at permissive (21°C) and non-permissive temperatures (31°C) were observed under light microscope by staining with different stains which were Toluidine Blue, Ruthenium Red and Calcofluor White…

Chromatography is a method of separation in which the components of a substance are separated and distributed between two phases, which are the mobile phases and stationary phase (1). There are multiple types of chromatograph, which includes paper chromatography, column chromatograph, retention chromatography, gas chromatography, liquid chromatography etc. Each technique has its own strengths and weaknesses, but all are useful in getting separations of different components (8). Since many…