Fluorescence microscope

the two strains of Tetrahymena were stained with mCherry fluorescence, so that the oral grooves could be examined and compared under fluorescence microscopy. This experiment was designed to eliminate as much bias as possible by replicating the experiment over 4 trials, one by each member of the team working on this project. Since this Sfr13 mutant strain has not been studied in depth, we chose the sample size this large to have replicated results that could be averaged for a more accurate representation of the population of Tetrahymena. Furthermore, the size was recorded to determine if that also correlated with the data being collected, which would eliminate the possibility that the correlation observed was due to size and not malformation of the oral groove. It was concluded that there is a correlation between the oral groove formation and feeding rate; however, other factors, such as size, would prevent the conclusion that the malformed oral grooved caused the Tetrahymena to feed slower. These two strains were chosen because…

An inverted fluorescence microscope is an inverted compound light microscope equipped with modules to internally separate the different wavelengths of light, and direct them to respective detection channels. Its main components include a light source, an objective, a filter cube and a detection unit, which can be an eyepiece or a camera (Fig.1). Filter cube is a component which separates a fluorescence microscope from a light microscope. It consists of one excitation filter, one emission filter…

EXERCISE 1 As Wenger and Potter (2015) assert, “stand-ups can serve several functions, so it’s important to know in advance what you’re trying to accomplish with an on-camera segment” (p. 284). Personally, I would need to prepare myself thoroughly and conduct some research of my own to learn more about the topic at hand. My goal as a reporter is to establish credibility on the subject and be able to project confidence to the audience (Wenger and Potter, 2015). According to Wenger and Potter…

There was an extra control slide so that the microscope could be adjusted without harming the samples. The cover slip was placed on top and nail polish was added to the sides of the cover slip to prevent the sample from drying out. With the 40x lens of the Zeiss Axiovision Widefield Fluorescence Microscope, images of the cells were captured. The two channels that were used were DAPI and DIC. DAPI had an exposure time of 960,000ms per picture and DIC had an exposure time of 7200ms per picture.…

endometrium of women who had endometriosis and those whom didn’t have it. The results of the two populations were compared. Due to this being done, it was shown that a particular protein known as DJ-1, is present and upregulated in eutopic endometrium of the woman surveyed with endometriosis. Therefore, it was inferred, that this protein may have impact or involvement of how endometriosis occurs. DJ-1 is a protein that is known to affect cell survival and growth as well as in ant oxidative…

of the polar amino acids of the protein interacting with the new created polarity in the phenyl ring. On the other hand, the polar C-F bond might also disturb non-polar interactions between the non-polar amino acids and the non-polar C-H bond; this would result in a lower equilibrium constant. It is clear from this experiment that the absorbance at 280 nm for BSA changes linearly between 100 and 200μM and becomes non-linear with increasing BSA concentration. This indicates severe concentration…

nanometers. UVC is far UV rays, and these rays go from 180 nanometers to 280 nanometers. There are a few times when radiation gets under this, all the way down to even ten nanometers, and are called extreme UV, but can only be created inside a vacuum because they are normally blocked by air. UV radiation has enough energy to break chemical bonds through a process called ionization. The higher energies of the radiation can cause the atoms to break away and form bonds that were not usually…

In this study, we developed an anthracene-based fluorescence PET sensor (AMG) was developed for detecting high pH values and water content in organic solvents. A guanidine group as a proton receptor was covalently attached to an anthracene fluorophore to afford highly polar AMG, with reasonable solubility in highly basic solution. The fluorescence of AMG increased almost linearly with decreasing pH in the range 11.0–13.0. AMG also exhibited a fluorescence turn-on response to water in various…

bacteria under the microscope with the resolutions 10x, 40x, and 100x with the use of immersion oil. Observations were noted and we replaced that yogurt slide with the prepared, stained yogurt slide. We compared and noted the observations between the two yogurt samples. Exercise 3: Preparing and Observing a Blood…

During the microscope lab, my partner and I learned how to properly use a microscope, calculate the field of view, and view and prepare slides. Microscopes are instruments used to view very small objects that cannot be seen with the naked eye. We found that microscopes have many different parts to them, including the diaphragm, ocular lens, and objective lenses. There are three powers of magnification, scanning power (4x), low power (10x), and high power (40x). To find the total magnification,…