Gram staining is a technique where certain stains are used to identify bacteria. It was discovered in 1884 by Hans Christian Gram where he used stains to differentiate bacterial cells in diseased lung tissue. Gram staining has evolved over time to become a trusted and common diagnostic tool used in clinical and research settings. Gram stains are mainly used to identify unknown bacteria. It is often the first test used on an unknown sample of bacteria. For medicinal uses gram staining is commonly…
Press lightly on the cover slip to rupture the spore casings but not the asci. 7. Place the slide on the microscope. 8. Using 10x objective, find an area of hybrid asci. 9. Increase the objective up to 40x. 10. Record the images on the slide. 11. Count at least 50 asci for each cross. 12. Record results. 13. Calculate the percentage of asci that show crossing over. 14. Calculate the recombination frequency. Hypothesis…
Microscopy is one of science’s greatest innovations. The microscope has revolutionised countless areas of scientific research and has become an icon of science itself. From Anton van Leeuwenhoek discovering micro-organisms in the 1670’s to the highly powerful atomic microscopes used today, microscopy has furthered scientific knowledge in many, many ways [1]. It has practical applications in very many areas of research such as biotechnology, materials science, microbiology, environmental science…
Introduction. Parts of light microscope. Steps to setup light microscope. Uses of light microscope. How to clean light microscope. Maintain the light microscope. Summery. References. Introduction. Light microscope is an instrument which used to magnify small specimen which cannot be seen through naked eye, by using a light source. It helps to magnify the small object to 1000* times than the real size of the real object. In light microscope light ray is send and it…
Wilson “Snowflake” Bentley was a scientist, atmospheric enthusiast, and very much a Vermonter in all aspects. From a very young age, he was interested in the “water wonders of the atmosphere.” In his hometown of Jericho, Vermont, he didn’t need to look far when pursuing his passion. His free time in the freezing months was spent isolated alone in the backyard. He didn’t mind though, and his determination to observe the beautiful crystalsled him to make some fantastic discoveries.…
Base: The base supports the microscope. (Microscope Master, 2016) (Human anatomy &Physiology Laboratory Manual, page 28) Describe universal precautions. Using information from current world events, locate and describe a situation that demonstrates why all healthcare professionals must be skilled…
In this lab I examined and drew different samples of slides and then categorized these slides into a logical evolutionary order. The first sample that most likely came first in the evolutionary order is the typical bacteria, which I viewed at a 1000 magnification. These bacteria can be found in the Eubacteria domain and kingdom. The bacteria in my sample were coccus, which means that the bacterium has a round shape, and this type of bacteria is one of the simplest forms of bacteria. These cells…
The Microscopic Identification of the Phases of Mitosis Using Onion Root Tip Cells Lauren Harvey and Jason Levigne Background: Mitosis is part one of the Mitotic Phase. The overall outcome the Mitotic Phase is taking one diploid cell (2n) and splitting it into two diploid (2n) cells. The Mitotic Phase includes two parts: Mitosis and Cytokinesis. The main goal of the Mitotic Phase is to achieve two things, both division of the genetic nuclear material, otherwise known as DNA, and the division of…
observe cell division in an onion root. I hypothesized that we would observe more cells in interphase since most of a cell’s life is spent in interphase. In order to make observations, we used a light microscope to view two slides of an onion root—one had been previously prepared while the other slide was made by each individual student. Furthermore, we then counted how many cells were in which phase and recorded our data. Our observations showed that our of the first 100 cell counted, 43 were…
the musty old smell in soil to E. coli which can cause food poisoning and kidney failure. The different bacterias present included gram positive bacteria and gram negative bacteria. Most of the gram positive bacteria was in clusters when under a microscope, found on the skin and in foods,may cause UTIs and acne. Most of the gram negative bacteria were rod shaped, found in the intestines, and feces and cause UTIs. It was found that there were many microorganisms in pedicure bowls and they all…