abnormal particles present from the skin or hair. Collecting the whole hair shaft is useful to see if the lice have infested themselves deeper in into the skin. Once you have obtained the hair sample, place it on a slide with the sample down, and proceed to examine it under the microscope (Cellophane Tape Method, 2013). 2) Another situation and species where a cellophane test would be useful is when trying to determine if a horse has pinworms. A louse examination is different…
Repeat step 4, 3 times after refocusing the microscope to see a different pane of bacteria. 6. Repeat steps 1 through 5 for each of the conical tubes. Study 2 - Lake Water Experiment 1. Repeat steps 4 through 9 from Study 1 if more of the stocks is needed. 2. Label 12 separate conical tubes with…
II. Method Concentration Gradients Experimet1: In this portion of the experiment, a beaker was obtained with 50mL of water, and it was sat until the water stopped moving. A small crystal of potassium permanganate dye was gently put into the water at the center of the beaker. Then the observation was recorded for the next 5 minutes. Experimet2: In this portion of the experiment, a beaker was obtained with 50mL of water in room temperature, and a teabag was placed into a beaker of water. The…
The slide was washed, blot, and examine under microspore. C. ACID FAST STAINING: The lipoid capsule of the acid – fast organism takes up carbolfuchsim and resists decolorizatin with a dilute acid rinse. The lipoid capsule of the mycobacterium is of such high…
described as gram-negative or gram-positive from conducting a gram stain. A bacterium that is gram-negative tends to have cells composed of a thin peptidoglycan layer, and a gram stain of gram-negative bacteria are seen as red or pink through a microscope (Smith and Hussey, 2005). On the other hand, a bacterium that is gram-positive will have cells made of a thick peptidoglycan layer, and the gram stain of a gram negative bacteria would be seen blue or purple (Smith and Hussey, 2005). In…
Using either a manual or automatic staining process, the immunoassay will identify the ALK target antigen on freshly prepared and fixed patient sample slides. After the localization of the antigen the antibody-antigen binding site will be made visible under a light microscope by a colored reaction. Methods to visualize will include a tertiary antibody attached to an enzyme that will find and bind to the secondary antibody which is anti-rabbit and bond to the primary…
University is the Philippine Red Cross - Manila Chapter. The Lacson Building where the laboratory is located is in between the University Gymnasium and Justo Albert Auditorium. The laboratory consists of several laboratory apparatus and most especially microscopes, which is greatly needed for the experimentation. Beverage samples will be collected from University Canteen, located in the University Activity Center, near the Gusaling Villegas Right Wing and PLM Pride Hall. B.…
objective was to carry out slide making techniques that will be a fundamental skill in future labs. If mitosis takes a relatively small time to carry out with comparison to the rest of the cell cycle, then more cells will most likely be found in interphase than being…
depression slide with grid lines. Vaseline was used to create a ring around the depression on the slide to prevent Stentor from seeping out. It was important to avoid applying Vaseline inside the depression of the slide as it may cause Stentor to become trapped. After completing the preparation to observe Stentor a pipette was used to extract three to four droplets of liquid that contained Stentor. Once the Stentor has been transferred from the pipette to the depression of the slide, a…
happen at the tips of the stems and roots. The region of elongation is the area in which growth occurs; this can restrict searches to the tips for growing plants like onion root tip to get the best results thin slice of the onion root placed on the slide stains the root. Examine under a…