Indicator

an unknown solution (analyte), titrant is used for identifying the concentration of that unknown by adding titrant to analyte. (using burette) Acid –base indicator: acid- base indicator is an indicator that determines the pH of a solution by changing its color due to H+ ion concentrations inside the solution. Indicator range: acid- base indicators change their colors when pH changes. However, they are useful in a specific pH interval. pH of a solution cannot be determined if pH is out of the…

were used to help understand the different behaviors of different concentrations. Household items were used to help apply basic theories about acids and bases such as pH. The titration curves were evaluated to help determine which indicator needed to be used for the indicator titration. Anions are ions that have more electrons than protons, therefore,…

KA to determine the identity. Another method used was qualitative methods. 3 indicators were set up with specific ranges of pH for colour change. Each of the indicators…

The purpose of this lab is to determine the Ka of an indicator within an unknown solution. This can be determined because acid-base indicators are weak bases or acids, and when neutralized they change colors. The color change is measured by the light absorption spectra in the compound which can be used to calculate the concentration. This experiment measures the concentrations by the different pH values which allows the pKa and Ka to be determined. Through the experiment, and the recorded color…

(NaOH) as a titrant. In addition, the measured volume of the base consumed is used to reach the end point. Hypothesis: It is expected to, at the end point of the experiment, the solution within the conical flask with both the acid, base, and indicator, will turn from a clear transparent color to a permanent pink color due to it being…

known concentration neutralizes an acid or base of unknown concentration. The neutralization of an acid or base in acid-base titration can be measured with either a color indicator or a pH-meter. For this experiment we are using a color indicator. The indicator phenolphthalein is used in experiment 1 and starch was use as an indicator in experiment 2.Phenolththalein is a colorless in acidic solution and pink in…

Pre-lab: An endpoint is indicated by some form of indicator at the end of a titration. The equivalence point is when the moles of a standard solution equal the moles of a solution of an unknown concentration. The endpoint of the solution should be a faint pink color. If the color is too dark of a pink that means that it is overly titrated. The independent variable is the amount of base being added to the solution. The dependant variable is the indicator. The constant variable is the acid…

Introduction: Microbiology is the study of all living microorganisms like bacteria, fungi, protozoans…etc, and non-living microorganisms like viruses, viroids and prions. The significance of identifying an unknown microorganism is first to understand what type of organism it is and what structures it has to help figure out their potential benefit, danger or even neutrality to humans. Physicians benefit from this by identifying what their patients are suffering from and how to treat them. Also…

products of each fermentation pathway. The Carbohydrate Fermentation Test was conducted in a two phenol red broth tubes that contained a fermentation broth that provided all the necessary nutrients for most microorganisms to grow, a phenol red pH indicator that changed colors at an acidic pH, and a specific…

Discussion: Each test is a pertinent contribution to drawing an educated conclusion. After performing the gram staining and observing which unknown culture was gram positive, a catalase test was performed. The catalase test allows lab workers to differentiate Staphylococci and Streptococci genus. The test was performed only on the gram-positive culture. Bacteria that respire by the use of oxygen, otherwise known as aerobic respiration, produce enzyme or “catalase”. This protects the bacteria…