Protein methods

month of September an article was published in an ‘Emergency Nurse’ journal. The heading of this article is “Protein Test May Ensure Quick Diagnosis of Heart Attack.” We all know that the odds of knowing when a heart attack may happen are very low. While a group of research nurses have come to the conclusion that you may be able to predict when a heart attack may happen by doing a simple protein test. Heart attacks can be fatal and hard to detect. This is what makes this discovery and hypothesis…

of a cell must be just right to sustain life and function. More demand would be placed on DNA for indefinitely growing cells because more protein production would be necessary for cellular function. Furthermore, more transcribed DNA to mRNA would lead to the RER working overtime to synthesize additional proteins. The ER would have to process a surplus of proteins being…

backbone of living cells to essentially perform the processes from cell division to death through the DNA, RNA, and protein information ways. More specifically, the central dogma defines the transfer of sequence information during DNA replication, transcription into RNA, and translation into amino-acid chains forming proteins. It also states that information cannot flow from protein to protein or nucleic acid. In addition, Hirao & Kimoto (2012) stated that: Through molecular biology studies,…

fibrosis affects ion transport in epithelial cells b) The CFTR protein regulates the transport of a number of ions; name 2 of these ions. (2 marks) The CFTR protein regulates the transport of Cl- and Na+ c) Explain why a defective CFTR gene could cause Dan to produce very salty sweat. (5 marks) The normal function of the CFTR gene provides instructions for making a protein channel called the cystic fibrosis transmembrane. This protein channel transports chloride ions across the semipermeable…

DNA ISOLATION INTRODUCTION: Deoxyribonucleic acid (DNA) isolation is an extraction process of DNA from various sources. Methods used to isolate DNA are dependent on the source, age, and size of the sample. Despite the wide variety of methods used, there are some similarities among them. In general, they aim to separate DNA present in the nucleus of the cell from other cellular components. Isolation of DNA is needed for genetic analysis, which is used for scientific, medical, or forensic purposes…

Determining the concentrations of proteins is necessary for a variety of different reasons. For example, protein concentration must be known in order for proteins to be used in enzyme reactions, as the correct stoichiometry must be known. Another example is protein purification; during protein purification the quantity of protein present must be known to determine the yield1. The quantification of proteins is determined by methods known as protein assays. There are different protein assays used…

the molecular weight of proteins within a sample. The protein sample was from the previous using anionic exchange chromatography to partially purify N-acetyl--D-hexosaminidase, Lab 3. The molecular weight was determined through the interpolation of a standard curve of proteins with a known molecular weight. The molecular weight of band 1 was determined to be 67,640 daltons and band 2 was 44,420 daltons, disproving the hypothesis that the molecular weight of the unknown protein sample was 28,000…

carbon dioxide (CO2) incubator. The next day, adding 10μM / ml puromycin effect for 24 hours, then infect JEV T1P1 strain for 24hr and 36hr. Final, collecting the cell (24 hours) and received supernatant (24 hours and 36 hours). Then a host protein and JEV protein were…

Protein is the building blocks of life (nlm.nih.gov). Amino Acids are the building block of protein. Amino acids bond together to make long chains, which is protein (nlm.nih.gov). Human body uses amino acids to make proteins to help the body break down food, repair body tissue, grow, and perform many other body functions (nlm.nih.gov). Amino acids can also used as a source of energy by the body (nlm.nih.gov). According to what I learned in Bioorganic lecture, the amino acid structure contains a…

Identifying the Presence of Macromolecules Using Benedict’s, Biuret’s, Sudan III, and Lugol’s Solution Betty Ngo Introduction Macromolecules are the critically important large molecules that fall into four main classes: carbohydrates, lipids, proteins and nucleic acids. Macromolecules are polymers which are built up by monomers. Monomers become polymers from the process dehydration reaction and polymers are broken apart by the process hydrolysis. Macromolecules are critically important…