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  • Protein Analysis Lab Report Protein

    Lab #5 Assignment: Protein Analysis by SDS-PAGE Introduction: Different cells and tissues express different proteins because of gene expression. Gene basically reflects what the protein will be and also its function. The expression must be controlled by some kind of regulation in the processes of transcription and translation. For example, in transcription, enhancer sequences provide binding sites for regulatory proteins that will affect RNA polymerase activity. On a related note, the size of proteins can be analyzed using SDS-PAGE (sodium dodecyl sulfate Polyacrylamide Gel). This technique is useful since it denatures and coats each protein being tested with the same charge, using β- mercaptoethanol and DTT. Gel- electrophoresis is then run to compare the sizes of different proteins. The goal of this experiment was to learn about the process of SDS-PAGE and also about gene expression by…

    Words: 863 - Pages: 4
  • Protein Solubility

    innate protein particles or aggregates.However, there is a mechanistic difference between the two aspects. In the case of a pure solubility issue, i.e. a protein becomes insoluble in a certain solution (no changes in protein structure and integrity) and a solid phase is formed, a strong dilution of the system may lead to the dissolution of the protein. In such a circumstance, the term “protein associates” may more appropriately describe the formed protein solid .In this instance, protein…

    Words: 833 - Pages: 4
  • Protein Lab

    Proteins Introduction Many products including powders, drinks, and foods are marketed as having more protein in them than the average consumption. But is that statement actually true? Proteins are a type of amino acid. There are twenty known amino acids monomers. Monomers are molecules that can be bonded together to create something else known as a polymer. Every amino acid has to have three things, an amino group, a carboxyl group and a functional group that is special to itself. Something…

    Words: 1039 - Pages: 5
  • Globular Proteins

    This writing presents the structure of proteins, their importance for life and processes under which they are made. It will also describe two groups of proteins and give some examples. Proteins (also called polypeptides, peptides or amino acids) are large polymers and main components of cells. They are produced by condensation reaction from amino acid monomers, therefore proteins are called condensation polymers. Each protein is formed by a unique sequence of 20 naturally occurring amino…

    Words: 795 - Pages: 4
  • Role Of Protein In Nutrition

    The Role of Protein in Nutrition There are 3 main micro-nutrients in food that we eat. Protein is one them and arguably the most important one for good health. Protein is used by many structures of the body, including muscle, to maintain and grow. Obviously the products in the table focus less on carbohydrates and fats, which are needed more for energy purposes. How We Use It So we get protein from food, but how do we actually use it to maintain the structure of our bodies? Our bodies break…

    Words: 1041 - Pages: 5
  • Protein Molecules Essay

    Proteins Molecules Building materials for cells. Cells have organelles such as mitochondria, lysosomes, vesicles, etc. It is estimated that the body has over ten trillions cells Carry oxygen, build tissue, copy DNA for the next generation and do the work needed by the body. Deoxyribonucleic acid contains the blueprint for proteins and replication of itself mRNA transcribes DNA, tRNA translates mRNA into proteins. A pattern on nucleotide on a DNA strand is called a gene. The Central Dogma of…

    Words: 805 - Pages: 4
  • Protein Analysis

    A protein has a precisely defined amino acid sequence which gives rise to its three dimensional structure which is essential to the protein’s function. These amino acids interact with one another to produce a specific folding pattern that produces the conformation of the protein. When one looks at the progression a protein makes in its folding complexity, it is clear that the folding is driven by the amino acid sequence alone. To understand this concept it is best to start with the primary…

    Words: 1207 - Pages: 5
  • Literature Review: Green Fluorescent Protein And Histidine Tagged Proteins

    Literature Review: Green Fluorescent Protein and Histidine Tagged Proteins Histidine and green fluorescent protein (GFP) tagging are important in molecular biology because they allow for purification, tracking, and quantification of target proteins (Ferrari et al., 2004; Cho et al., 2011; Deponte, 2012). Histidine tagging provides a method for isolating and increasing the amount of target protein recovered from a biological organism or mixed sample of proteins (Masek et al., 2011; Singh and…

    Words: 1537 - Pages: 6
  • Protein Synthesis Paper

    This paper is going to describe the replication of DNA and RNA and the processes of transcription and translation of protein synthesis. What is DNA? DNA is a nucleic acid that carries the genetic information in cells and some viruses, consisting of two long chains of nucleotides twisted into a double helix and joined by hydrogen bonds between the complementary bases adenine and thymine or cytosine and guanine. DNA sequences are replicated by the cell prior to cell division and may include genes,…

    Words: 400 - Pages: 2
  • Protein And Starchloric Acid Experiment

    group tried to answer was “Which protein solution contained protein, starch, and sugar, and if so how much?” The protein supplements we had were supplments 4 (Kroger Whey & Soy Protein- Vanilla),6 (Vega Protein and Greens) ,and 8 (Pure Protein Natural Whey Protein). We also had 3 reagents (Biuret’s, Lugol’s ,and Benedict’s). We had two different experiments, the first experiment was to get qualitative data. The second was to get quantitative date. The first experiment we tested each supplement…

    Words: 855 - Pages: 4
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