Lab #5 Assignment: Protein Analysis by SDS-PAGE
Introduction:
Different cells and tissues express different proteins because of gene expression. Gene basically reflects what the protein will be and also its function. The expression must be controlled by some kind of regulation in the processes of transcription and translation. For example, in transcription, enhancer sequences provide binding sites for regulatory proteins that will affect RNA polymerase activity. On a related note, the size of proteins can be analyzed using SDS-PAGE (sodium dodecyl sulfate Polyacrylamide Gel). This technique is useful since it denatures and coats each protein being tested with the same charge, using β- mercaptoethanol and DTT. Gel- electrophoresis is then run to compare the sizes of different proteins. The goal of this experiment was to learn about the process of SDS-PAGE and also about gene expression by …show more content…
Figure 2 showed a visual of Figure 1, comparing relative mobility and molecular weight. It can be seen more clearly that as the Rf value increased, the more the molecular weight decreased. 14.4 kD had the highest Rf of 0.926, compared to 94 kD with an Rf of 0.211. Figure 2: The semi-logarithmic graph above showed the relationship between relative mobility and molecular weight. Figure 3 showed the actual picture of the gel and its bands, noting the most important ones. Using Figure 2’s regression line, the most important bands’ molecular weight was calculated. Bands 1, for B samples, ended up being 49.4 kD, along with band 2 in C sample. For both samples of D, bands 3 were both 34.6 kD, along with band 5. The molecular weight for band 4 ended up being 43.4 kD. Band 6 represented 63 kD.
Picture of Gel Figure 3: Shown is the results from the gel-electrophoresis for each of the samples.
Introduction:
Different cells and tissues express different proteins because of gene expression. Gene basically reflects what the protein will be and also its function. The expression must be controlled by some kind of regulation in the processes of transcription and translation. For example, in transcription, enhancer sequences provide binding sites for regulatory proteins that will affect RNA polymerase activity. On a related note, the size of proteins can be analyzed using SDS-PAGE (sodium dodecyl sulfate Polyacrylamide Gel). This technique is useful since it denatures and coats each protein being tested with the same charge, using β- mercaptoethanol and DTT. Gel- electrophoresis is then run to compare the sizes of different proteins. The goal of this experiment was to learn about the process of SDS-PAGE and also about gene expression by …show more content…
Figure 2 showed a visual of Figure 1, comparing relative mobility and molecular weight. It can be seen more clearly that as the Rf value increased, the more the molecular weight decreased. 14.4 kD had the highest Rf of 0.926, compared to 94 kD with an Rf of 0.211. Figure 2: The semi-logarithmic graph above showed the relationship between relative mobility and molecular weight. Figure 3 showed the actual picture of the gel and its bands, noting the most important ones. Using Figure 2’s regression line, the most important bands’ molecular weight was calculated. Bands 1, for B samples, ended up being 49.4 kD, along with band 2 in C sample. For both samples of D, bands 3 were both 34.6 kD, along with band 5. The molecular weight for band 4 ended up being 43.4 kD. Band 6 represented 63 kD.
Picture of Gel Figure 3: Shown is the results from the gel-electrophoresis for each of the samples.