Protein methods

causes the harmful effects in tuberculosis. The bacterium spreads throughout the body through methods similar to protein synthesis. However, certain antibiotics such as, streptomycin, help prevent and cure cases of TB. The antibiotics work through altering or interfering in the processes of replication, transcription, and translation. Streptomycin is a specific anti-biotic that helps inhibit the protein synthesis of the bacterium, essentially declining the rate of growth and killing off the rest…

Phosphonates as inhibitors for Pks13-TE Hypothesis for the aim 1 of the proposed project is that the fluorinated α–Aminophosphonates as a covalent irreversible inhibitor for the Mtb Pks13-TE by scission of the P-C bond. Previous studies have shown the fluorinated aminophosphonates (FAPs) demonstrated serine esterases inhibition via scission of the P-C bond as shown in Figure 2.1.A 4. FAPs are known to irreversibly inhibit serine esterases such as acetylcholinesterase16 , neuropathy target…

3.1 1. The improvements in the microscope help scientists see the cells better and distinguish the different organelles inside the cell. 2. Prokaryotic cells do not have a nucleus and most internal structures that eukaryotic cells. 3. This method builds on the work of early scientists by changing the soup broth with bacteria in it to a preserved petri dish. 4. Both cells and atoms are basic building blocks. 5. This poison would not kill bacteria because bacteria do not have a nucleus. 3.2 1.…

act the same, some directly bind to amino acids, while others relay the signals indirectly through protein interactions. Identifying FLCN amino acid reservoir allows us to determine how FLCN interacts with amino acids by providing insight whether the interaction is direct or…

1.5. Enzyme-Polysaccharide Interactions Proteins and enzymes have become an integral part of many industrial processes as well as being applied both in cosmetics and in therapeutics. Due to advances in biotechnology and genetic engineering, mass production of protein and enzymes has become feasible. Use of enzymes is environmentally friendly as they catalyse reactions under mild conditions against polluting inorganic catalysts which works in the extremes of pH and temperature. However, this can…

Corporation 's have lied to you about consumers and minimum wage workers being the backbone to their industry. Enzymes are. Enzymes are biological protein catalysts that encourage chemical reactions by lowering the activation energy needed for the reaction to take place. Once the reaction is complete, enzymes are left unchanged and able to move on to the next reaction. For this reason, enzymes have proven to be valuable resources for industries. The reusability of enzymes are especially useful…

Assignment: Protein Analysis by SDS-PAGE Introduction: Different cells and tissues express different proteins because of gene expression. Gene basically reflects what the protein will be and also its function. The expression must be controlled by some kind of regulation in the processes of transcription and translation. For example, in transcription, enhancer sequences provide binding sites for regulatory proteins that will affect RNA polymerase activity. On a related note, the size of proteins…

of inner membrane of eggshell of chicken Gallus gallus domesticus. Fresh samples of egg shells are cleaned; dried and inner membrane of the shell is separated carefully for FTIR analysis. FTIR spectrum reveals a series of bands related to fats and proteins. Calcium carbonate is the major constituent (about 90%) of the shell, but it is absent in the inner shell membrane as characteristic band at 712 cm-1 is not seen in the spectrum. However, characteristic bands at 870 cm-1 and 1450 cm-1 suggest…

Her-2/neu Test HER2/neu is a protein found on the surface of all breast cells. HER2/neu plays a role in normal cell growth. It also is found on the surface of some breast cancer cells. This means the tumor is HER2/neu-positive. A breast cancer cell that has a high level of HER2/neu can make breast cancer more likely to grow and spread. Sometimes these types of tumors can also be more aggressive (overexpression). They also can respond differently to treatment than HER2/neu-negative tumors. You…

collected. Protein Extraction and Quantification The absorbance vs concentration curve prepared from the Bradford Protein Assay standards was found to have a linear regression of y = 2.2184x + 0.0626 as shown in Figure 1. The absorbances of the liver, muscle, and adipose tissue were used to determine the protein concentrations of the samples. The percent protein was found to be 15.64% in liver tissue, 4.13% in muscle…